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miRNA Reagents

miRNAprep Pure FFPE Kit

Highly efficient extraction of RNA > 18 nt from paraffin sections

Catalog number / packaging

Cat. no

ID

No. of preps

4992863

 DP502 50 preps
Manual Inquire Inspection report

  • Storage

    DNase I, buffer RDD and RNase-Free ddH2O (tube) should be stored at 2-8°C. Other reagents should be stored at room temperature (15-25°C).

  • Description

    Formalin fixation can lead to cross-linking of RNA-RNA and RNA- protein in tissues, thus affecting the application of RNA in downstream experiments. The kit provides unique lysis and incubation conditions to reverse the crosslinking of RNA caused by formalin. After xylene deparaffinization, RNA can be effectively released from tissue sections by treatment with specially developed lysis buffer. The digestion of DNase I can ensure no genomic DNA contamination. RNA > 18 nt can be obtained after purification by silica matrix membrane. The product can be directly used in downstream experiments such as RT-PCR, RT-qPCR, Microarray analysis, etc.

  • Features

    ■ High specificity: Specially designed for extracting miRNA from FFPE with optimized components.

    ■ Simple and fast: High-quality miRNA can be obtained within 2 hours.

    ■ Ultra-pure: Column purification to avoid gDNA and salt contamination.

  • Required Reagents

    Xylene, anhydrous ethanol

  • Applications

    ■ RT-PCR

    ■ RT-qPCR

    ■ Northern Blot analysis

    ■ Microarray analysis

    ■ Sequencing of miRNA library

  • Use TIANGEN miRNAprep Pure FFPE Kit to extract miRNA of the paraffin sections of rat liver with a thickness of about 10 μm, and then sequentially carry out reverse transcription and fluorescence quantitative PCR to detect the target miRNA. A: Extract two sections and detect the expression of miR-16. Set up 2 repetitions for each section. The amplification curve shows that the kit can efficiently and stably extract miRNA in paraffin section samples. B: Use TIANGEN miRNAprep Pure FFPE Kit and relevant products from Supplier A and B to extract miRNA from the tissue sections. Each kit extracts two sections and all sections are from the same tissue sample. After extraction, perform reverse transcription and detect miR-194 expression. The experimental results in the figure show that the extraction yield of TIANGEN miRNAprep Pure FFPE Kit is slightly higher than that of similar kits of Supplier A and B.

  • Use TIANGEN miRNAprep Pure FFPE Kit to extract miRNA of the paraffin sections of rat liver with a thickness of about 10 μm, and then sequentially carry out reverse transcription and fluorescence quantitative PCR to detect the target miRNA. A: Extract two sections and detect the expression of miR-16. Set up 2 repetitions for each section. The amplification curve shows that the kit can efficiently and stably extract miRNA in paraffin section samples. B: Use TIANGEN miRNAprep Pure FFPE Kit and relevant products from Supplier A and B to extract miRNA from the tissue sections. Each kit extracts two sections and all sections are from the same tissue sample. After extraction, perform reverse transcription and detect miR-194 expression. The experimental results in the figure show that the extraction yield of TIANGEN miRNAprep Pure FFPE Kit is slightly higher than that of similar kits of Supplier A and B.

Publication

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