Single Column

EndoFree Maxi Plasmid Kit

For purification of endotoxin-free transfection grade plasmid DNA

Catalog number / packaging

Cat. no	ID	No. of preps
4992194	DP117	10

Manual Inquire

Storage

Room temperature (15-25℃ )

Room temperature (15-25℃ )
Description

The EndoFree Maxi Plasmid Kit is based on silica membrane technology to extract endotoxin-free plasmid DNA. This kit provides a unique endotoxin removal process to generate high-quality plasmid DNA for downstream applications. It is designed to isolate plasmid DNA of the highest purity from 100-200 ml of bacterial culture and could yield up to 1.5 mg transfection grade plasmid DNA.

EndoFree Maxi Plasmid Kitはシリカメンブレン法に基づいて、エンドトキシンフリーのプラスミドDNAを抽出します。本キットは、繊細な下流のアプリケーションで使用するためのエンドトキシンフリーな高品質プラスミドDNAを精製するための独自の手法を提供します。本キットは100～200mLの細菌培養液から最高純度のプラスミドDNAを単離するために設計されており、1.5mgの形質転換グレードのプラスミドDNAを精製可能です。
Features

■ Fast and high yield: 200 μg-1.5 mg plasmid DNA yield in 1 hour with high proportion of super-coiled structure.

■ High-purity: High-purity plasmid DNA are acquired by unique buffer system and Spin Column CP6.

■ Excellent transfection efficiency: Suitable for transfection experiments of most cell lines.

■ Wide range of applications: Suitable for restriction enzyme digestion, transformation, sequencing, microinjection, gene silencing and transfection experiments.

■１時間で200 µg -1.5 mg のプラスミドDNAが得られ、スーパーコイル構造を保持します。

■独自のバッファーシステムとSpin Column CP6により、低エンドトキシンレベルと高純度のプラスミドDNAを保証します。

■優れたトランスフェクション効率で、ほとんどの細胞株のトランスフェクション実験に使用できます。

■制限酵素消化、形質転換、シークエンス、遺伝子治療、ライブセルイメージング、遺伝子サイレンシング、トランスフェクション実験など幅広いアプリケーションに適しています。

Experimental Example

High/low copy plasmid extraction

Low-copy plasmid pBR322, extracted from 200 ml bacterial culture using EndoFree Maxi Plasmid Kit, was eluted in 1 ml Buffer TB, with a concentration of 0.6 μg/μl. High-copy plasmid pBS, extracted from 100 ml bacterial culture using EndoFree Maxi Plasmid Kit ,was eluted in 1 ml Buffer TB, with a concentration of 1.2 μg/μl.

MIV: TIANGEN DNA Marker IV

pBR322: 2 μl, pBS : 2 μl

High/low copy plasmid extraction

Low-copy plasmid pBR322, extracted from 200 ml bacterial culture using EndoFree Maxi Plasmid Kit, was eluted in 1 ml Buffer TB, with a concentration of 0.6 μg/μl. High-copy plasmid pBS, extracted from 100 ml bacterial culture using EndoFree Maxi Plasmid Kit ,was eluted in 1 ml Buffer TB, with a concentration of 1.2 μg/μl.

MIV: TIANGEN DNA Marker IV

pBR322: 2 μl, pBS : 2 μl
pEGFP obtained with EndoFree Maxi Plasmid Kit were separately transfected into endotoxin-insensitive cell line 293T and endotoxin-sensitive cell line Huh7 cells by TIANGEN TIANfect. Expression of GFP were detected in 24 hours after transfection.

pEGFP obtained with EndoFree Maxi Plasmid Kit were separately transfected into endotoxin-insensitive cell line 293T and endotoxin-sensitive cell line Huh7 cells by TIANGEN TIANfect. Expression of GFP were detected in 24 hours after transfection.

Single Column

EndoFree Maxi Plasmid Kit

Catalog number / packaging

Storage

Description

Features

Experimental Example

Publication