DNA Library Kits/Modules
-
-
TIANseq Fast Ligation Module
Fast and efficient ligase system
Catalog number / packaging
Cat. no
ID
No. of preps
4992355
NG303-02 96 rxn 4992354
NG303-01 24 rxn
Powered by Bioz
Inquire-
Storage
This kit should be stored at-25~-15°C. -
Description
TIANSeq Fast Ligation Module is a premixed enzyme module specifically optimized for Illumina high-throughput sequencing platform. Using this module, the DNA fragment with dA-tailing at the 3' end can be quickly ligated with adapter. Compared with the conventional method, this module adopts a one-step reaction process. The DNA fragments obtained after the reaction of TIANSeq Fragment/ Repair/Tailing Module or TIANSeq End Repair/dA-Tailing Module can be directly ligated with the adapter without magnetic bead purification, thus eliminating multi-step magnetic bead purification steps, making the operation simpler and the library conversion efficiency higher. -
Required Reagents
TIANSeq Fragment /Repair /Tailing Module (Cat# 4992350/4992351)
TIANSeq End repair/dA-Tailing Module (Cat# 4992352/4992353)
TIANSeq NGS Library Amplification Module (Cat #4992373/4992374)
TIANSeq Single-Index Adapter (Illumina) (Cat# 4992641/4992642/4992378)
TIANSeq Size Selection DNA Beads (Cat# 4992358/4992359)
-
Features
■ The DNA adapter can be directly ligated with the DNA fragment with dA-Tailing without the need for magnetic bead purification.
■ High efficient ligation, which can be used for DNA adapter ligation of trace samples.
-
Applications
Fast ligation of DNA adapter with DNA library fragment with dAtailing at the 3' end. Applicable sample input: 0.25 ng~1 μg DNA.
