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RNA Library Kits/Modules

TIANSeq rRNA Depletion Kit (H/M/R)

Rapidly and efficiently depletion of ribosomal RNA, which increase the proportion of effective sequencing data

Catalog number / packaging

Cat. no

ID

No. of preps

4992391

 NR101-03 96 rxn

4992364

 NR101-02 24 rxn

4992363

 NR101-01 6 rxn
Manual Inquire Inspection report

  • Storage

    This kit should be stored at-15~-25°C.

  • Description

    TIANSeq rRNA Depletion Kit (H/M/R) uses a specially designed DNA probe to hybridize with ribosomal RNA(rRNA), and then uses Ribo RNase H to degrade rRNA in the DNA-rRNA hybrid chain, thus removing cytoplasmic ribosomal RNA(28S, 18S, 5.8S, 5S) and mitochondrial ribosomal RNA(16S, 12S) in human, mouse and rat total RNA, and retaining messenger RNA(mRNA) and other non-coding RNA.

    The kit has a good rRNA removal effect on complete and partially degraded total RNA (such as FFPE RNA).The obtained rRNA-removed RNA sample can be used for mRNA and non-coding RNA high-throughput sequencing. The effective data ratio in sequencing results can be obviously improved, and the purified product can also be used for random primer cDNA synthesis or other downstream applications.

  • Required Reagents

    TIANSeq RNA Clean Beads (Cat#4992360/4992362/4992867)

    Ethanol

  • Features

    ■ Wide sample range: Suitable for depletion of rRNA in high quality (complete) and partially degraded (e.g. FFPE) samples.

    ■ Efficient removal: 95-99.9% of human/mouse/rat rRNA is effectively removed.

    ■ Comprehensive data: incomplete mRNA and non-coding RNA information are retained, making transcriptome data more comprehensive.

    ■ Rapid evaluation: The primers provided in the kit can quickly evaluate the removal effect of rRNA.

  • Applications

    This kit is suitable for the construction of RNA transcriptome library. It can also be used for random primer cDNA synthesis or other downstream applications.

Experimental Example

  • Agilent 2100 results show that rRNA removal is complete for high quality (intact) and partially degraded samples.

  • NGS sequencing results showed that rRNA is completely removed.

    The sequencing result of total RNA of mouse liver shows that before using the kit to remove rRNA, the reads of control group accounted for more than 85% of the total Mapped reads, while the reads of rRNA after TIANSeq rRNA Depletion Kit (H/M/R) removal accounted for less than 0.2%.The sequencing result of total RNA of mouse liver shows that before using the kit to remove rRNA, the reads of control group accounted for more than 85% of the total Mapped reads, while the reads of rRNA after TIANSeq rRNA Depletion Kit (H/M/R) removal accounted for less than 0.2%.

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