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Extraction Kits for Automatic Instruments

TGuide Smart Magnetic Plant RNA Kit

Purify high-quality total RNA from plant tissues

Catalog number / packaging

Cat. no

ID

No. of preps

4993552(cartridge)

 DP662-DE 48
Manual Inquire Inspection report

  • Reagent Selection Guide for TGuide S16



    Nuclei Acid Sample Type Product Name

    Packing size (preps)

    Cartridge/plate

    Cat no.

    Cartridge/plate

    DNA Animal Tissue
    		 TGuide Smart Magnetic Tissue DNA Kit 48/96
    		 4993547/4995038
    DNA Plant & Seed TGuide Smart Magnetic Plant DNA Kit 48 4993548
    DNA Soil/Stool TGuide Smart Soil /Stool DNA Kit 48 4993549
    DNA Gel & PCR products TGuide Smart DNA Purification Kit 48 4993550
    DNA  Whole Blood TGuide Smart Blood Genomic DNA Kit 48/96
    		 4993703/4995206
    DNA Cell/Swab/Dry Spots,etc TGuide Smart Universal DNA Kit 48/96
    		 4993704/4995040
    RNA Blood/Cell/Tissue TGuide Smart Blood/Cell/Tissue RNA Kit 48/96
    		 4993551/4995043
    RNA Plant & Seed TGuide Smart Magnetic Plant RNA Kit 48 4993552
    DNA/RNA Cell Free Fluids (Serum,etc) TGuide Smart Viral DNA/RNA Kit 48/96
    		 4993702/4995207


  • Product introduction

    •Purify high-yield, high-purity, high-quality total RNA from plant tissues.

    •No toxic reagents such as phenol/chloroform contained.

    •The RNA obtained has high purity and can be directly used for chip detection, high-throughput sequencing and other experiments.

  • Features

    Super easy to use.



    No extra pipetting work.

    The elution volume can be adjusted as required.


    The installation and operation require little training. With pre-installed programs, unpack the cartridge, select the protocol and run your experiment. Programs are ready-to-use, and also customizable.



    Preloaded reagents and matched disposable consumables.

    The chemical handling is eliminated to the greatest extent to ensure the operation safety.



  • Wheat root total RNA extraction

    Sample size: 100 mg

    Sample pretreatment: grinding with liquid nitrogen or low temperature tissue homogenizer treatment

    Agarose gel concentration :2%(TAE)

    Loading volume: 2 μl

    Marker: Marker IV, TIANGEN



    Experiment result: using TGuide S16 achieved with reagent 4993552 to automatically extract the total RNA of wheat roots has good yield and high purity. In this experiment, about 20 μg of nucleic acid was extracted from 100 mg wheat roots, with OD260/OD280 around 2.0~2.1, and OD260/OD230＞2.0.


  • Total RNA extraction from different plant samples

    Sample size: 100 mg

    Sample pretreatment: low temperature homogenizer

    Agarose gel concentration :1% (TAE)

    Loading volume: 1 μl

    M: Marker III, TIANGEN

    1-4: corn seeds 5-8: corn leaves 9-12: cabbage roots 13-16: soybeans

    The first two samples were extracted by spin-column extraction kit and the last two samples were extracted by TGuide S16.



    Experiment result: the extraction yield of plant RNA by TGuide S16 with reagent 4993552 is equivalent to or better than that by the spin-column extraction kit. The purity

    and integrity are basically the same. Thus, TGuide S16 can be used to extract plant total RNA in replacement of the spin-column based extraction solution.


Publication

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