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Single Column

TIANamp Bacteria DNA Kit

Rapid extraction of high quality genomic DNA from various Gram-negative, Gram-positive bacteria.

Catalog number / packaging

Mat. No

Ref. No

No. of preps

4992448

 GDP302-02 50
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  • Features

    ● Simple and fast: High purity genomic DNA of Gram-negative bacteria can be obtained within 1 hour.

    ● Excellent quality: The purifed DNA can be directly used in downstream molecular experiments such as PCR, restriction endonuclease digestion, Southern blotting, etc.

  • Description

    The TIANamp Bacteria DNA Kit adopts a highly efficient, DNA-specific adsorption spin column and a unique bufer system for both genomic DNA extraction from Gram-negative and Gram-positive bacteria. The kit can also be used for the extraction of pathogenic bacteria (microorganisms) of food, such as Staphylococcus aureus, cholera sclerotia, hemorrhagic Escherichia coli O157:H7, Listeria monocytogenes, Salmonella, Enterobacter sakazakii, etc. 10-40 μl of pure genomic DNA can be rapidly extracted and purifed from 1-5 ml of bacterial culture medium, and the obtained genomic DNA can be directly used in molecular biology experiments such as PCR template, restriction enzyme digestion and Southern blot.

  • Kit Contents

  • Applications

    ● Genome extraction of Gram-negative bacteria.

    ● Genome extraction of Gram-positive bacteria.

    ● Genome extraction of pathogenic bacteria in food.

  • Required Reagents

    Lysozyme (Gram-positive bacteria), ethanol, lysozyme buffer (20 mM Tris, pH8.0; 2 mM Na2-EDTA, 1.2% Triton-100).

  • Yield For Reference

    Note: The DNA extraction amount may vary depending on the bacteria types and culture time, etc. Gram-positive bacteria require special treatments such as lysozyme for lysing, and the genomic DNA extraction can be performed according to the procedures of Gram-negative bacteria.

  • Storage Condition

    TIANamp Bacteria DNA Kit should be kept in dry place and can be stored at room temperature (15-30°C) for up to 15 months without showing any reduction in performance and quality. If any precipitate forms in the buffers, it should be dissolved by warming the buffers at 37°C for 10 min before use.

  • Important Notes

    ● Repeated freezing and thawing of stored samples should be avoided, since this leads to DNA size reduction.

    ● If precipitates formed in Buffer GA or Buffer GB, warm the buffer to 37°C until the precipitates have fully dissolved.

    ● All centrifugation steps should be carried out in a conventional table-top centrifuge at room temperature (15-30°C).

  • Experimental Example

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