Single Column
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Storage
Room temperature (15-25℃) -
Description
The TIANamp Bacteria DNA Kit adopts a highly efficient, DNA-specific adsorption spin column and a unique buffer system for both genomic DNA extraction from Gram-negative and Gram-positive bacteria. The kit can also be used for the extraction of pathogenic bacteria (microorganisms) of food, such as Staphylococcus aureus, cholera sclerotia, hemorrhagic Escherichia coli O157:H7, Listeria monocytogenes, Salmonella, Enterobacter sakazakii, etc. 10-40 μl of pure genomic DNA can be rapidly extracted and purified from 1-5 ml of bacterial culture medium, and the obtained genomic DNA can be directly used in molecular biology experiments such as PCR template, restriction enzyme digestion and Southern blot. -
Required Reagents
Lysozyme (Gram-positive bacteria), ethanol, lysozyme buffer (20 mM Tris, pH8.0; 2 mM Na2-EDTA, 1.2% Triton-100) -
Features
■ Simple and fast: High purity genomic DNA of Gram-negative bacteria can be obtained within 1 hour
■ Excellent quality: The purified DNA can be directly used in downstream molecular experiments such as PCR, restriction endonuclease digestion, Southern blotting, etc.
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Applications
■ Genome extraction of Gram-negative bacteria
■ Genome extraction of Gram-positive bacteria
■ Genome extraction of pathogenic bacteria in food
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DNA Extraction Yield From Various Plant Tissues:
Note: The DNA extraction amount may vary depending on the bacteria types and culture time, etc. Gram positive bacteria require special treatments such as lysozyme for lysing, and the genomic DNA extraction can be performed according to the procedures of Gram-negative bacteria.
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