PCR (Polymerase Chain Reaction) is an in vitro nucleic acid amplification technology. Its basic principle is enzymatic DNA synthesis reaction, that is, in the presence of template DNA, primers and deoxyribonucleic acid, the DNA fragments are cyclically replicated and rapidly amplified by the catalysis of thermostable DNA polymerase through the steps of DNA strand thermal denaturation, primer annealing and primer extension.

TIANGEN’s has developed various one-tube PCR reaction system (PCR MasterMix), each of which is divided into dye-containing and dye-free systems. Dye-containing MasterMix products can be directly electrophoresed after PCR, without loading buffer. The specificity and sensitivity of PCR reaction are obviously improved, and complex templates with high GC content and secondary structure can be effectively amplified. As low as 2 copies of the target template can be amplified, making the experimental results more accurate. The original MasterMix formula makes the whole reaction system very stable and will not affect the activity even if it is frozen and thawed for many times or kept at 4℃ for a long time. The stable and efficient pre-prepared PCR MasterMix is fast and simple, greatly reducing labor intensity and sampling error. The high-performance PCR enhancer and optimizer are added to enhance the PCR reaction.