Column-based Method

Column-based Method

To obtain nucleic acid, the basic steps include: cell lysis—nucleic acid extraction—nucleic acid washing—elution. After the cell lysis step, the RNA extraction can be performed either with organic solvent extraction or matrix adsorption method. The principle of using RNA silica-membrane technology to achieve RNA separation and purification is mainly to utilize the binding of nucleic acid to silica matrix material under high salt conditions and detachment under low salt conditions. The mechanism is that high concentration of salt ions destroy the molecular structure of silica matrix and water, forming a cationic, so that the nucleic acid is easily bound to the silica matrix; when the salt is washed away, the silica can be rehydrated, and the rehydrated silica destroys the attraction between the matrix and the nucleic acid. RNA can then be eluted from the silica membrane by RNase-Free deionized water.

The TIANGEN column-based RNA extraction kits can effectively remove the Impurities such as proteins and organic solvents with optimized buffer, such as Buffer RW1, and high-purity RNA can thus be obtained very conveniently.

  • TIANamp Virus RNA Kit

    Professional virus RNA purification kit

  • RNAsimple Total RNA Kit

    For the high-efficient total RNA extraction using the widely used centrifugal column

  • RNAprep Pure Micro Kit

    For purification of high-quality total RNA from micro amount of tissues or cells

  • DNA/RNA Isolation Kit

    For the purification of both DNA and RNA from the same sample

  • RNAprep Pure Cell/Bacteria Kit

    For purification of high-quality total RNA from cells and bacteria

  • RNAprep Pure Tissue Kit

    For purification of up to 100 μg total RNA from animal tissues

  • RNAprep pure FFPE Kit

    For purification of RNA from formalin-fixed, paraffin-embedded tissues

  • RNA Easy Fast Tissue/Cell Kit

    For purification of high-quality total RNA from animal tissues/cells

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