● Targeted: It is specially formulated for plant samples that are difficult to extract, such as polysaccharides and polyphenolics-rich plants. The process is more optimized, and the results are reliable.

● Efficient removal of gDNA: High-efficient DNase I is supplied for rapid removal of gDNA on the column.

● Easy and fast: RNA extraction experiments can be completed within 1 hour.

● Safe and low toxicity: No toxic organic reagents such as phenol and chloroform are needed.

RNAprep Pure Plant Plus Kit provides a fast, simple, and cost-effective method for purification of total RNA from plant cells and tissues, especially from plant tissues rich in polysaccharides, polyphenolics and starch, such as cotton leaves, mature rice leaves, Arabidopsis thaliana seeds, white pine needles, banana, loquat leaves, potato tubers, apple, pear, watermelon, kiwi fruit, Chinese rose, tobacco, sea-buckthorn, lily, etc. The purified RNA is ready for use in downstream applications such as RT-PCR and real-time RT-PCR, microarray, northern blot, dot blot, polyA screening, in vitro transcription, and molecular cloning.

RNase-Free DNase I, Buffer RDD and RNase-Free ddH2O should be stored at 2-8°C for 15 months; Buffer SL/β-mercaptoethanol mix can be stored at 2-8°C for 1 month; Others stored at room temperature (15-30°C) for 15 months. 

● Add β-Mercaptoethanol (β-ME) to Buffer SL before use. The final concentration of β-ME is 5%. For example, add 25 μl β-ME to 475 μl Buffer SL. Buffer SL with β-ME can be stored at 2-8°C for up to one month. Buffer SL may form precipitate upon storage. If necessary, redissolve it by warming.

● RNAprep Pure Plant Plus Kit provides a choice of Buffer HL. Buffer SL can be used for most plant samples. However, for tissues with special secondary metabolites (such as milky endosperm of maize, red bean or wheat seeds) or filamentous fungi, guanidine thiocyanate in Buffer SL may cause sample solidification, which will affect RNA extraction. In this condition, TIANGEN provides an alternative lysis buffer HL.

● Buffer RW is supplied as a concentrate. Before using for the first time, ethanol (96-100%) should be added as indicated on the bottle to obtain a working solution.