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Column-based Method

RNAprep Pure Plant Plus Kit

For purification of total RNA from polysaccharides & polyphenolics-rich plant samples

Catalog number / packaging

Cat. no

ID

No. of preps

4992239

 DP441 50
Manual Inquire Inspection report

  • Storage

    DNase I, Buffer RDD, RNase-Free ddH2O (Tubular) should be stored at 2-8℃

    Other reagents could be stored at room temperature (15-25℃)

  • Description

    The RNAprep Pure Plant Plus Kit (Polysaccharides & Polyphenolics-rich) is a silicon matrix purification RNA extraction kit developed for multiple plant samples with polysaccharides and polyphenolics. It is supplied with Buffer SL with superb lysis ability. High-purity total RNA without gDNA can be extracted from the fleshes of bananas, watermelons, apples, pears, the tubers of sweet potatoes, potatoes, as well as the leaves of cotton, rose, alfalfa, rice and white pine needles within 1 hour.

  • Required Reagents

    β-mercaptoethanol, ethanol

  • Features

    ■ Targeted: It is specially formulated for plant samples that are difficult to extract, such as polysaccharides and polyphenolics-rich plants. The process is more optimized, and the results are reliable.

    ■ Efficient removal of gDNA: High-efficient DNase I is supplied for rapid removal of gDNA on the column.

    ■ Easy and fast: RNA extraction experiments can be completed within 1 hour.

    ■ Safe and low toxicity: No toxic organic reagents such as phenol and chloroform are needed.

  • Applications

    This kit can be directly used for various molecular biology experiments such as RT-PCR, real time PCR, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis, and cloning, etc.

Experimental Example

  • Total RNA was extracted from 100 mg fleshes of banana, watermelon, apple and pear, tubers of sweet potato and potato, leaves of cotton, rose, alfalfa, rice and white pine needles respectively using RNAprep Pure Plant Plus Kit. 4-6 μl of 30 μl eluates were loaded per lane.


    M: TIANGEN Marker III;

    The electrophoresis was conducted at 6 V/cm for 30 min on a 1% agarose.

    Results: RNAprep Pure Plant Plus Kit can extract high purity, high yield and  good integrity total RNA from the polysaccharides & polyphenolics-rich plant samples.


  • Total RNA was extracted from 100 mg fleshes of banana, watermelon, apple and pear, tubers of sweet potato and potato, leaves of cotton, rose, alfalfa, rice and white pine needles respectively using RNAprep Pure Plant Plus Kit. 4-6 μl of 30 μl eluates were loaded per lane.

    M: TIANGEN Marker III;

    The electrophoresis was conducted at 6 V/cm for 30 min on a 1% agarose.

    Results: RNAprep Pure Plant Plus Kit can extract high purity, high yield and  good integrity total RNA from the polysaccharides & polyphenolics-rich plant samples.






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