Column-based Method
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Storage
DNase I, Buffer RDD, RNase-Free ddH2O (Tubular) should be stored at 2-8℃
Other reagents could be stored at room temperature (15-25℃)
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Description
The RNAprep Pure Plant Plus Kit (Polysaccharides & Polyphenolics-rich) is a silicon matrix purification RNA extraction kit developed for multiple plant samples with polysaccharides and polyphenolics. It is supplied with Buffer SL with superb lysis ability. High-purity total RNA without gDNA can be extracted from the fleshes of bananas, watermelons, apples, pears, the tubers of sweet potatoes, potatoes, as well as the leaves of cotton, rose, alfalfa, rice and white pine needles within 1 hour. -
Required Reagents
β-mercaptoethanol, ethanol -
Features
■ Targeted: It is specially formulated for plant samples that are difficult to extract, such as polysaccharides and polyphenolics-rich plants. The process is more optimized, and the results are reliable.
■ Efficient removal of gDNA: High-efficient DNase I is supplied for rapid removal of gDNA on the column.
■ Easy and fast: RNA extraction experiments can be completed within 1 hour.
■ Safe and low toxicity: No toxic organic reagents such as phenol and chloroform are needed.
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Applications
This kit can be directly used for various molecular biology experiments such as RT-PCR, real time PCR, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis, and cloning, etc.
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