DNA methylation is a covalent modification of DNA bases, which exists in all higher organisms. DNA methylation can turn off the activity of some genes, while demethylation can induce the reactivation and expression of genes, thus leading to the change of gene expression. DNA methylation plays an important role in maintaining normal cell function, genetic imprinting, embryonic development and human disease. With the development of methylation research, a variety of methylation detection methods have been developed to meet the requirements of different types of research. These methods can be divided into three categories: the detection of the overall level of methylation, the verification of the methylation of gene specific sites and the search for new methylation sites.
TIANGEN provides the complete set of products for DNA bisulfate conversion, Methylation specific PCR (MSP) and well as High resolution melting (HRM) analysis.
Methylation-speciﬁc PCR (MSP) Kit
Methylation-specific PCR detection kit
HRM Analysis Kit (EvaGreen）
Professional reagent for high resolution melting curve analysis
DNA Bisulfite Conversion Kit
he conversion and purifi cation can be completed in 2 hour, with the conversion rate up to 99%