RT-PCR is a technique that combines reverse transcription (RT) of RNA with polymerase chain amplification (PCR) of cDNA. Firstly, cDNA is synthesized from RNA by reverse transcriptase, and then cDNA is used as template to amplify the synthesized target fragment. RT-PCR technology is sensitive and widely used. It can be used for detecting gene expression level in cells, RNA virus content in cells and directly cloning cDNA sequences of specific genes.
According to different experimental requirements, TIANGEN has developed various RT products with different characteristics, such as the reverse transcription reagent FastKing RT Kit, which can completely remove gDNA residue, and TIANScript II RT Kit, which is especially suitable for long template transcription. Users can choose according to specific experimental requirements.