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Magnetic Beads-based Method

Magnetic Viral DNA/RNA Kit

Highly efficient purification of viral nucleic acid from serum, plasma, lymph, cell-free body fluid and urine

Catalog number / packaging

Cat. no

ID

No. of preps

4992408

 DP438-01 50

4992409

 DP438-02 200

4992915

 DP438-03 1000
Manual Inquire Inspection report

  • Storage

    The kit can be stored for 12 months under dry conditions at room temperature (15-25°C). and stored at 2-8°C for a longer time.

  • Description

    The kit adopts magnetic beads with unique separation function and a unique buffer system to separate and purify high-quality virus DNA/RNA from serum, plasma, lymph, cell-free body fluid, cell culture supernatant, urine or various virus preservation solutions. Unique embedded magnetic beads have strong affinity for nucleic acid under certain conditions. When the conditions change, the magnetic beads release adsorbed nucleic acid, thus achieving the purpose of fast separation and purification of nucleic acid. The whole process is safe and convenient. With high yield extracted virus DNA/RNA, high purity, stable and reliable quality, it is especially suitable for automatic extraction of high-throughput workstations. The nucleic acid purified by the kit can be suitable for various conventional operations, including various downstream experiments such as RT-PCR, real time PCR, etc.

  • Required Reagents

    Isopropanol, ethanol.

  • Features

    ■ Simple and fast: High-quality viral DNA or RNA can be obtained within 1 hour.

    ■ High throughput: Can be integrated with magnetic rod method automatic instrument or pipetting method automatic instrument to carry out high throughput extraction experiment.

    ■ Safe and nontoxic: No toxic organic reagents such as phenol/chloroform are needed.

Experimental Example

  • Mean Ct value detected by real-time PCR

    After extracting RNA of AIV-H5 (10-6, 10-7, 10-8 dilution gradient) using TIANGEN Magnetic Viral DNA/RNA Kit and relevant product from Supplier T respectively, the virus RNA was detected by real-time PCR using TIANGEN SuperReal PreMix plus. The results show that compared with the product of Supplier T, TIANGEN Magnetic Viral DNA/RNA Kit has lower Ct value, and the yield is slightly higher, especially for low concentration samples.

  • After extracting RNA of AIV-H5 (10-6, 10-7, 10-8 dilution gradient) using TIANGEN Magnetic Viral DNA/RNA Kit and relevant product from Supplier T respectively, the virus RNA was detected by real-time PCR using TIANGEN SuperReal PreMix plus. The results show that compared with the product of Supplier T, TIANGEN Magnetic Viral DNA/RNA Kit has lower Ct value, and the yield is slightly higher, especially for low concentration samples.
  • After extracting RNA of AIV-H5 (10-6, 10-7, 10-8 dilution gradient) using TIANGEN Magnetic Viral DNA/RNA Kit and relevant product from Supplier T respectively, the virus RNA was detected by real-time PCR using TIANGEN SuperReal PreMix plus. The results show that compared with the product of Supplier T, TIANGEN Magnetic Viral DNA/RNA Kit has lower Ct value, and the yield is slightly higher, especially for low concentration samples.

Publication

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