● Optimized buffers and protocols for cultured cells and bacteria samples make the process simple and convenient.

● Unique DNase I minimizes genomic DNA contamination.

● Unique RNase-Free Filtration Columns CS eliminates other contaminations.

● The high-purity and ready-to-use RNA is suitable for sensitive downstream applications.

● No phenol/chloroform extraction, no LiCl and ethanol precipitation, no CsCl gradient centrifugation are needed, which makes the process safe and reliable.

● RT-PCR.

● Northern Blot, Dot Blot.

● Real-Time PCR.

● Chip analysis.

● PolyA Screening, in vitro translation, RNase protection analysis and molecular cloning.

β-mercaptoethanol, ethanol, lysozyme (optional for bacterial RNA extraction)

DNase I, Buffer RDD, RNase-Free ddH2O (Tubular) should be stored at 2-8℃ Other reagents could be store at room temperature (15-30℃). 

● β-Mercaptoethanol (β-ME) must be added to Buffer RL before use. The final concentration of β-ME is 1%. For example, add 10 μl β-ME to 1 ml Buffer RL. Buffer RL containing β-ME can be stored at 2-8°C for 1 month. Buffer RL may form a precipitate upon storage. If necessary, re-dissolve by warming, and then place at room temperature (15-30°C).

● Buffer RW is supplied as a concentrate. Before using for the first time, add ethanol (96-100%) as indicated on the bottle to obtain a working solution.

● Perform all steps in RT if not indicated, centrifugation steps at 15-30°C in a standard centrifuge.

● Do not recommend storage sample in buffer with lysing capacity.