Column-based Method

RNAprep Pure Cell/Bacteria Kit

For purification of high-quality total RNA from cells and bacteria

Catalog number / packaging

Cat. no	ID	No. of preps
4992235	dp430	50

Manual Inquire

Storage

DNase I, Buffer RDD, RNase-Free ddH₂O (Tubular) should be stored at 2-8℃

Other reagents could be stored at room temperature (15-25℃)

DNase I, Buffer RDD, RNase-Free ddH2O (Tubular) should be stored at 2-8℃

Other reagents could be stored at room temperature (15-25℃)
Description

The RNAprep Pure Cell/Bacteria Kit provides a fast, simple and cost-effective method for purification of total RNA from cultured cells and bacteria samples by using effective spin column and unique buffer system. The kit includes RNase-Free Spin Column CR3 for purifying high quality RNA by using silica-membrane technology. High-quality total RNA could be obtained in 30-40 minutes with high-purity and is free of protein and genomic DNA contamination.

RNAprep Pure Cell/Bacteria Kitは独特なバッファーシステムと効果的なシピンカラムを採用し、様々な種類の培養細胞から迅速にトータルRNAを抽出することができ、同時に大量の異なるサンプルを処理することができます。本キットは高品質なRNAを30～40分で精製可能です。精製したRNAはタンパク質による汚染が無く高純度です。
Required Reagents

β-mercaptoethanol, ethanol, lysozyme (optional for bacterial RNA extraction)

β-mercaptoethanol, ethanol, lysozyme (optional for bacterial RNA extraction)
Features

■ Optimized buffers and protocols for cultured cells and bacteria samples make the process simple and convenient.

■ Unique DNase I minimizes genomic DNA contamination.

■ Unique RNase-Free Filtration Columns CS eliminates other contaminations.

■ The high-purity and ready-to-use RNA is suitable for sensitive downstream applications.

■ No phenol/chloroform extraction, no LiCl and ethanol precipitation, no CsCl gradient centrifugation are needed, which makes the process safe and reliable.

■培養細胞とバクテリアに最適化され、操作が簡単である。

■効果的なDNaselはDNAコンタミネーションが効果的に除去できる。

■Filtration Columns CSが付いているので、他の不純物が効果的に除去できる。

■RNAの純度がより高く、不純物の残留がなく、特に純度に高い要求のある下流の試験に適している。

■操作が安全で、フェノール/クロロホルム抽出が不要で、塩化セシウム勾配遠心、塩化リチウムやエタノール沈殿必要がない。
Applications

■ RT-PCR.

■ Northern Blot, Dot Blot.

■ Real-Time PCR.

■ Chip analysis.

■ PolyA Screening, in vitro translation, RNase protection analysis and molecular cloning.

■ RT-PCR.

■ Northern Blot, Dot Blot.

■ Real-Time PCR.

■ Chip analysis.

■ PolyA Screening, in vitro translation, RNase protection analysis and molecular cloning.

Experimental Example

Material: Human Jurkat Cells (1×10⁶ )

Method: The total RNA of Human Jukat Cells was isolated using the RNAprep Pure Cell/Bacteria Kit.

Results: Please see the above agarose gel electrophoresis picture. 2-4 μl of 50 μl eluates were loaded per lane. The electrophoresis was conducted at 6 V/cm for 30 min on 1% agarose gel.

Material: Human Jurkat Cells (1×106 )

Method: The total RNA of Human Jukat Cells was isolated using the RNAprep Pure Cell/Bacteria Kit.

Results: Please see the above agarose gel electrophoresis picture. 2-4 μl of 50 μl eluates were loaded per lane. The electrophoresis was conducted at 6 V/cm for 30 min on 1% agarose gel.
Material: TOP10 E.coli (1×10⁸)

Method: The total RNA of TOP10 E.coli was isolated using the RNAprep Pure Cell/Bacteria Kit.

Results: Please see the above agarose gel electrophoresis picture. 2-4 μl of 50 μl eluates were loaded per lane. The electrophoresis was conducted at 6 V/cm for 30 min on 1% agarose gel.

Material: TOP10 E.coli (1×108)

Method: The total RNA of TOP10 E.coli was isolated using the RNAprep Pure Cell/Bacteria Kit.

Results: Please see the above agarose gel electrophoresis picture. 2-4 μl of 50 μl eluates were loaded per lane. The electrophoresis was conducted at 6 V/cm for 30 min on 1% agarose gel.

Column-based Method

RNAprep Pure Cell/Bacteria Kit

Catalog number / packaging

Storage

Description

Required Reagents

Features

Applications

Experimental Example

Publication