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Column-based Method

RNAprep Pure Cell/Bacteria Kit

For purification of high-quality total RNA from cells and bacteria

Catalog number / packaging

Mat. No

Ref. No

No. of preps

4992235

 Gdp430 50
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  • Storage

    DNase I, Buffer RDD, RNase-Free ddH2O (Tubular) should be stored at 2-8℃

    Other reagents could be stored at room temperature (15-25℃)

  • Description

    The RNAprep Pure Cell/Bacteria Kit provides a fast, simple and cost-effective method for purification of total RNA from cultured cells and bacteria samples by using effective spin column and unique buffer system. The kit includes RNase-Free Spin Column CR3 for purifying high quality RNA by using silica-membrane technology. High-quality total RNA could be obtained in 30-40 minutes with high-purity and is free of protein and genomic DNA contamination.

  • Required Reagents

    β-mercaptoethanol, ethanol, lysozyme (optional for bacterial RNA extraction)

  • Features

    ■ Optimized buffers and protocols for cultured cells and bacteria samples make the process simple and convenient.

    ■ Unique DNase I minimizes genomic DNA contamination.

    ■ Unique RNase-Free Filtration Columns CS eliminates other contaminations.

    ■ The high-purity and ready-to-use RNA is suitable for sensitive downstream applications.

    ■ No phenol/chloroform extraction, no LiCl and ethanol precipitation, no CsCl gradient centrifugation are needed, which makes the process safe and reliable.

  • Applications

    ■ RT-PCR.

    ■ Northern Blot, Dot Blot.

    ■ Real-Time PCR.

    ■ Chip analysis.

    ■ PolyA Screening, in vitro translation, RNase protection analysis and molecular cloning.

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