Column-based Method
InquireCatalog Number｜Packaging
Mat. No |
Ref. No |
No. of preps |
|
4992235 |
GDP430 | 50 |
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Features
● Optimized buffers and protocols for cultured cells and bacteria samples make the process simple and convenient.
● Unique DNase I minimizes genomic DNA contamination.
● Unique RNase-Free Filtration Columns CS eliminates other contaminations.
● The high-purity and ready-to-use RNA is suitable for sensitive downstream applications.
● No phenol/chloroform extraction, no LiCl and ethanol precipitation, no CsCl gradient centrifugation are needed, which makes the process safe and reliable.
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Description
The RNAprep Pure Cell/Bacteria Kit provides a fast, simple and costeffective method for purification of total RNA from cultured cells and bacteria samples by using effective spin column and a unique buffersystem. The kit includes RNase-Free Spin Column CR3 for purifying high-quality RNA by using silica-membrane technology. High-quality total RNA could be obtained in 30-40 minutes with high-purity and is free of protein and genomic DNA contamination.
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Kit Contents
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Applications
● RT-PCR.
● Northern Blot, Dot Blot.
● Real-Time PCR.
● Chip analysis.
● PolyA Screening, in vitro translation, RNase protection analysis and molecular cloning.
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Required Reagents
β-mercaptoethanol, ethanol, lysozyme (optional for bacterial RNA extraction)
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Storage Condition
DNase I, Buffer RDD, RNase-Free ddH2O (Tubular) should be stored at 2-8℃ Other reagents could be store at room temperature (15-30℃).
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Important notes
● β-Mercaptoethanol (β-ME) must be added to Buffer RL before use. The final concentration of β-ME is 1%. For example, add 10 μl β-ME to 1 ml Buffer RL. Buffer RL containing β-ME can be stored at 2-8°C for 1 month. Buffer RL may form a precipitate upon storage. If necessary, re-dissolve by warming, and then place at room temperature (15-30°C).
● Buffer RW is supplied as a concentrate. Before using for the first time, add ethanol (96-100%) as indicated on the bottle to obtain a working solution.
● Perform all steps in RT if not indicated, centrifugation steps at 15-30°C in a standard centrifuge.
● Do not recommend storage sample in buffer with lysing capacity.
Experimental Example
Material: Human Jurkat Cells (1×106 )
Method: The total RNA of Human Jukat Cells was isolated using the RNAprep Pure Cell/Bacteria Kit.
Results: Please see the above agarose gel electrophoresis picture. 2-4 μl of 50 μl eluates were loaded per lane. The electrophoresis was conducted at 6 V/cm for 30 min on 1% agarose gel.
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