Column-based Method
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Features
● Convenient: DNA and RNA can be purified simultaneously from the same sample.
● Fast: DNA/RNA extraction can be completed within 40-50 min.
● Safe: No phenol/chloroform is required for the extraction process.
● Reliable: The purified DNA/RNA can be used in various downstream experiments.
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Description
The DNA/RNA Isolation Kit allows rapid and simultaneous extraction of DNA and total RNA from cultured animal cells or tissues, and large numbers of different samples can be processed simultaneously. The reaction can be completed within 40-50 minutes. The extracted DNA and total RNA are of high purity, and can be used in various molecular biology downstream experiments such as PCR and RT-PCR, etc.
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Kit Contents
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Applications
Applicable for various downstream molecular experiments such as PCR and RT-PCR, etc.
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Required Reagents
β-mercaptoethanol, ethanol -
Storage Condition
Store at room temperature (15-30℃). -
Important notes
● Add β-mercaptoethanol (β-ME) to Buffer RLplus to a final concentration of 1% before use. For example, add 10 μl β-mercaptoethanol (β-ME) per 1 ml Buffer RLplus. Buffer RLplus may form precipitate during storage. If necessary, redissolve by warming it in 56°C, and then equilibrate to room temperature.
● Before start, add ethanol (96-100%) to Buffer RW, Buffer PW and Buffer GD for a working solution, as described on the bottle.
● The following operations were carried out at room temperature unless otherwise indicated.
● For some sensitive RNA samples, genomic DNA may need to be removed completely in the following application. Please refer to DNase I digestion process with column.
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