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Features
● The high-purity ready-to-use RNA is suitable for sensitive downstream applications.
● Wide applications: The purified RNA can be applied to various experimental samples.
● The experiment can be completed in 1 hour with simple operations.
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Description
The RNAsimple Total RNA Kit adopts a new RNA extraction method based on guanidinium isothiocyanate/phenol method. The Buffer RZ specifically designed by TIANGEN can remove genomic DNA and proteins from cells rapidly and efficiently, making the obtained RNA highly pure and stable.
This kit is used to separate total RNA from blood, animal cells, tissues, and plant tissues. Each spin column can process 50-100 mg tissue or 5x106 cells at a time, and can process a large number of different samples simultaneously. The reaction can be completed in less than an hour, and the total RNA extracted has a higher yield, better purity, with no DNA and protein contamination, and can be used in various downstream experiments.
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Kit Contents
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Applications
● RT-PCR
● Northern Blot, Dot Blot.
● Real-Time PCR
● PolyA Screening, in vitro translation, RNase protection analysis, molecular cloning.
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Required Reagents
Ethanol, chloroform -
Storage Condition
Store at room temperature (15-30°C).
Buffer RZ should be stored at 2-8°C away from light.
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Important notes
For isolation of bacterial RNA, RNAprep Pure Cell/Bacteria Kit should be used (Cat.no. 4992235).
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