Column-based Method

RNAsimple Total RNA Kit

For the high-efficient total RNA extraction using the widely used centrifugal column

Catalog number / packaging

Cat. no


No. of preps


DP419 50
  • Storage

    Store at room temperature (15-25℃)

    Buffer RZ should be stored at 2-8℃ away from light

  • Description

    The RNAsimple Total RNA Kit adopts a new RNA extraction method based on guanidinium isothiocyanate/phenol method. The Buffer RZ specifically designed by TIANGEN can remove genomic DNA and proteins from cells rapidly and efficiently, making the obtained RNA highly pure and stable.

    This kit is used to separate total RNA from blood, animal cells, tissues, and plant tissues. Each spin column can process 50-100 mg tissue or 5x106 cells at a time and can process a large number of different samples simultaneously. The reaction can be completed in less than one hour, and the total RNA extracted has a higher yield, better purity, with no DNA and protein contamination, and can be used in various downstream experiments.

  • Required Reagents

    Ethanol, chloroform
  • Features

    ■ The high-purity ready-to-use RNA is suitable for sensitive downstream applications.

    ■ Wide applications: The purified RNA can be applied to various experimental samples.

    ■ The experiment can be completed in 1 hour with simple operations.

  • Applications

    ■ RT-PCR

    ■ Northern Blot, Dot Blot.

    ■ Real-Time PCR

    ■ PolyA Screening, in vitro translation, RNase protection analysis, molecular cloning.

Experimental Procedure

  • Experimental Procedure
  • Material: 20 mg rat spleen tissue

    Method: The total RNA of rat spleen tissue was isolated using the RNAsimple Total RNA Kit.

    Results: Please see the above agarose gel electrophoresis picture. 2-4 μl of 100 μl eluates were loaded per lane. The electrophoresis was conducted at 6 V/cm for 30 min on a 1% agarose.