Column-based Method
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Features
● High-quality virus RNA can be obtained within one hour.
● No phenol/chloroform extraction and ethanol precipitation are needed.
● High RNA yield and excellent repeatability.
● Complete removal of contaminants and inhibitors, facilitating downstream applications.
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Description
The TIANamp Virus RNA Kit provides a fast, simple and cost-effective virus RNA purification method for virus RNA purification from whole blood, plasma, serum and other cell-free materials by using effective spin column and unique buffer system. This kit is supplied with carrier RNA to enhance the binding of nucleic acid to the spin column membrane. The obtained RNA is free of protein and nuclease contamination and can be used directly in hybridization, RT-PCR, and one step qPCR experiments. TIANamp Virus RNA Kit is suitable for various kinds of virus RNA purification and high-quality RNA can be obtained within one hour.
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Kit Contents
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Applications
● Virus genotyping research.
● Virus epidemiologic study.
● Virus infectious diseases analysis.
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Required Reagents
Ethanol, DNase I (optional) -
Storage Condition
Store at room temperature (15-30℃).
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Important notes
● All protocol steps should be carried out at room temperature (15-30°C).
● Equilibrate the samples to room temperature.
● RNase-Free Centrifuge tubes 1.5 ml are used in step 14. Others are not supplied.
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