Column-based Method
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Features
● Silica membrane based technology to ensure high-purity of RNA.
● Fast and simple process compared with conventional methods.
● Suitable for downstream RT-PCR or RT-qPCR applications.
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Description
The RNAprep Pure FFPE Kit is specially designed for purifying total RNA from formalin-fixed, paraffin-embedded tissue sections. Special lysis and incubation conditions can remove formaldehyde modifications of RNA. In addition, the lysis buffer efficiently releases RNA from tissue sections while avoiding further RNA degradation. The kit also uses DNase I and Buffer RDD for optimized removal of genomic DNA contamination. The purified RNA can be used in various of down stream applications such as RT-PCR. -
Kit Contents
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Applications
This kit is suitable for the purification of total RNA from formalin-fixed, paraffin-embedded tissue sections (FFPE).
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Required Reagents
Xylene, ethanol -
Storage Condition
DNase I, Buffer RDD, RNase-Free ddH2O (Tubular) should be stored at 2-8℃. Other reagents can be stored at room temperature (15-30℃).
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Important notes
● Add ethanol (96-100%) to Buffer RW before start as indicated on the bottle.
● Preparation of DNase I stock solution: Dissolve the lyophilized DNase I (1500 U) in 550 μl of the RNase-Free ddH2O. Mix gently by inverting. Do not vortex. Divide it into single-use aliquots, and store at -30~-15°C for up to 9 months.
● Note: Thawed aliquots from -30~-15°C could be stored at 2-8°C for up to 6 weeks. Do not refreeze the aliquots after thawing.
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