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Column-based Method

RNAprep pure FFPE Kit

For purification of RNA from formalin-fixed, paraffin-embedded tissues

Catalog number / packaging

Cat. no

ID

No. of preps

4992303

 dp439 50
Manual Inquire Inspection report

  • Storage

    DNase I, Buffer RDD, RNase-Free ddH2O (Tubular) should be stored at 2-8℃.

    Other reagents could be store at room temperature (15-25℃)

  • Description

    The RNAprep Pure FFPE Kit is specially designed for purifying total RNA from formalin-fixed, paraffin-embedded tissue sections. Special lysis and incubation conditions can remove formaldehyde modifications of RNA. In addition, the lysis buffer efficiently releases RNA from tissue sections while avoiding further RNA degradation. The kit also uses DNase I and Buffer RDD for optimized removal of genomic DNA contamination. The purified RNA can be used in various of downstream applications such as RT-PCR.

  • Required Reagents

    Xylene, ethanol

  • Features

    ■ Silica membrane based technology to ensure high-purity of RNA.

    ■ Fast and simple process compared with conventional methods.

    ■ Suitable for downstream RT-PCR or RT-qPCR applications.

  • Applications

    This kit is suitable for the purification of total RNA from formalin-fixed, paraffin-embedded tissue sections (FFPE).

Experimental Example

  • RNA extracted from paraffin-embedded rat liver sample using the RNAprep Pure FFPE Kit.

    Sample amount: 15 mg rat liver; Elution volume: 50 μl; Loading volume: 8 μl

    M: TIANGEN Marker III

    1-4: Rat liver FFPE

Publication

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