• Simple and fast: Ultra-pure total DNA can be obtained by running TGuide S16 within 1 hr.

• Safe and non-toxic: No toxic reagents such as phenol/chloroform.

• Strong commonality: It can be used to extract genomic DNA from blood, tissues, swabs, bacteria, cells and other samples.

• High purity: The DNA obtained has high purity and can be directly used for chip detection, high-throughput sequencing and other experiments.

The kit adopts magnetic beads and a unique buffer system, to isolate and purify genomic DNA with high quality from blood, dried blood spot, bacteria, cells, saliva, oral swabs, animal tissues and other samples. The uniquely embedded magnetic beads have a strong affinity for nucleic acid under certain conditions. When the conditions are changed, the magnetic beads can release the absorbed nucleic acid to rapidly separate and purify it. The whole process is safe and convenient, and the extracted genomic DNA has large fragments and high purity and is stable and reliable in quality. It can perfectly fit with TGuide S16 Nucleic Acid Extractor of TIANGEN for automated extraction.

DNA purified by this kit is suitable for downstream experiments including enzymatic digestion, PCR, qPCR, library construction, Southern Blot, chip analysis and high-throughput sequencing.

The kit can be stored in dry conditions at room temperature (15~30°C) for 12 months. If the solution has precipitation, it can be incubated in 37°C water bath for 10 min to dissolve the precipitation, without affecting the effect.

● To use the TGuide Smart Universal DNA Kit, you must have the TGuide Smart Universal DNA (program no. DP605-01/02) installed on the TGuide S16/S32 Nucleic Acid Extractor.

● The sample should avoid repeated freezing and thawing, otherwise the DNA fragments extracted will be small and the amount of extraction will be reduced.

● If there is precipitation in the Buffer GHA, it can be re-dissolved in 37°C water bath and used after mixing.

● The pre-processing methods of different samples can be different. Please read the manual carefully before the experiment and prepare the reagent by users.

● If you need a TGrinder H24R tissue homogenizer for electric homogenization of tissue, users need to purchase and ask TIANGEN for the grinding method.

● After sample pre-processing, take an appropriate amount of the sample and add it to Column1/7 of the 96-Deepwell plate.

● There are two automated extraction programs in the operational steps of this kit, Pause program DP605-01 and Non-stop program DP605-02. Wherein, DP605-01 is recommended to use in nucleic acid extraction from blood samples, and the instrument will automatically pause after the end of step 6. At this moment, users need to add 350 µl isopropanol in Column1/7 of the 96-Deepwell plate. Besides, if the sample is cells and users have a high demand for the purity of cell genomic DNA, it is also recommended to use the program DP605-01. Non-stop program DP605-02 is recommended to use in the nucleic acid extraction from other samples. Before the 96-Deepwell plate operation, users need to add 350 µl isopropanol in the Column1/7, and then run the DP605-02 program.

● The elution buffer is not prefilled, users need to add the elution buffer to the Column 5/11 of the 96-Deepwell plate before performing on the instrument. The recommended range of elution buffer is 60~100 µl. The smaller the elution volume, the higher the nucleic acid concentration, but the lower the total yield may be. On the contrary, the larger the elution volume, the lower the nucleic acid concentration, and the higher the total yield may be. You can adjust it in the range of 60~100 µl as needed.