Menu
English

English

中文

Janpanese (Japan)

Korean (Korea)

miRNA Reagents

miRcute miRNA Isolation Kit

For high effective extraction of small fragment RNA at the range of 20-200 nt

Manual Inquire Inspection report Trial

Catalog Number｜Packaging

Mat. No

Ref. No

No. of preps

4992860

 GDP501 50

  • Features

    ● Efficient and accurate separation of small RNA fragments at the sizes of 20-200 nt.

    ● Can be applied on small fragment RNA extraction as well as total RNA extraction.

    ● Easy to operate, all operations can be completed in 1 hour.

    ● High purity, the extracted RNA is free of DNA and protein contamination, facilitating the analysis of downstream experiments.

  • Description

    The miRcute miRNA Isolation Kit is a miRNA extraction and isolation kit based on the adsorption column method. It can efficiently extract small RNAs such as miRNA, siRNA, snRNA, etc., which are 20-200 nt in length, and can also extract total RNA. The efficient lysis buffer can easily lyse various tissues and cells. The adsorption column uses a special silicon matrix membrane packing, which greatly enhances its ability to adsorb RNA, especially small RNA (<200 nt).

  • Kit Contents

  • Applications

    ● Northern Blot analysis

    ● Real-Time PCR

    ● Microarray analysis

  • Required Reagents

    Ethanol, chloroform

  • Storage Condition

    Buffer MZ should be stored at 2-8°C and protected from light for 15 months. Other solutions and columns should be stored dry at room temperature (15-30°C) for 15 months.

  • Important Notes

    To avoid RNase contamination, please note that:

    ● Change gloves regularly. Bacteria on the skin can result in RNase contamination.

    ● Use RNase-Free plastic and tips to avoid cross contamination.

    ● RNA can be protected in Buffer MZ. But RNA must be stored or applied in RNase-Free plastic or glassware. To wipe off RNase, the glassware can be heated at 150°C for 4 hours, and plastic can be dipped in 0.5 M NaOH for 10 min, washed by RNase-Free ddH2O thoroughly, and sterilized.

    ● Use RNase-Free ddH2O to prepare solution. (Add DEPC to 0.1% final concentration in ddH2O. Shake solution to mix, and leave overnight at room temperature, autoclave for 15 min).

Experimental Example

The Ct value of the miRNA extracted from 30 mg mouse liver using miRcute miRNA Isolation Kit was smaller than that of traditional isopropanol precipitation method.

Sort by

Date Date()

Date Date()

Impact Factor IF()

Impact Factor IF()

Inquire