miRNA Reagents
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Features
● Efficient and accurate separation of small RNA fragments at the sizes of 20-200 nt.
● Can be applied on small fragment RNA extraction as well as total RNA extraction.
● Easy to operate, all operations can be completed in 1 hour.
● High purity, the extracted RNA is free of DNA and protein contamination, facilitating the analysis of downstream experiments.
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Description
The miRcute miRNA Isolation Kit is a miRNA extraction and isolation kit based on the adsorption column method. It can efficiently extract small RNAs such as miRNA, siRNA, snRNA, etc., which are 20-200 nt in length, and can also extract total RNA. The efficient lysis buffer can easily lyse various tissues and cells. The adsorption column uses a special silicon matrix membrane packing, which greatly enhances its ability to adsorb RNA, especially small RNA (<200 nt). -
Kit Contents
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Applications
● Northern Blot analysis
● Real-Time PCR
● Microarray analysis
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Required Reagents
Ethanol, chloroform -
Storage Condition
Buffer MZ should be stored at 2-8°C and protected from light for 15 months. Other solutions and columns should be stored dry at room temperature (15-30°C) for 15 months. -
Important Notes
To avoid RNase contamination, please note that:
● Change gloves regularly. Bacteria on the skin can result in RNase contamination.
● Use RNase-Free plastic and tips to avoid cross contamination.
● RNA can be protected in Buffer MZ. But RNA must be stored or applied in RNase-Free plastic or glassware. To wipe off RNase, the glassware can be heated at 150°C for 4 hours, and plastic can be dipped in 0.5 M NaOH for 10 min, washed by RNase-Free ddH2O thoroughly, and sterilized.
● Use RNase-Free ddH2O to prepare solution. (Add DEPC to 0.1% final concentration in ddH2O. Shake solution to mix, and leave overnight at room temperature, autoclave for 15 min).
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Experimental Example
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