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miRNA Reagents

miRcute Plus miRNA First-Strand cDNA Kit

Faster and more sensitive reagent for reverse transcription of miRNA by tailing method

Catalog number / packaging

Mat. No

Ref. No

No. of preps

4992786

 GKR211-01 20 µl×25 rxn

4992909

 GKR211-02 20 µl×50 rxn
Manual Inquire Inspection report

  • Features

    ● Time-saving and labor-saving: A-tailing reaction and reverse transcription reaction is combined in one step, reducing the operation steps and saving half of the reaction time.

    ● Strong specificity: Specific modification and reverse transcription of single-strand miRNA to avoid the influence of miRNA precursor with secondary structure.

    ● High sensitivity: All miRNA A-tailing products are used for further reverse transcription reaction, greatly improving the detection rate of lowly abundant miRNA.

    ● Widely applicable: Reverse transcription reaction can be carried out on miRNA extracted from almost all materials. The input range of templates can reach 20 pg-2 µg (mass range) and 10 fM-100 pM (concentration range).

  • Description

    The miRcute Plus miRNA cDNA First-Strand cDNA Kit adopts the A-tailing method to carry out reverse transcription of miRNA first strand cDNA. The kit adopts a two-component form, thus simplifying the experimental operation process and reducing the possibility of operation errors. miRNA RT Enzyme Mix in this product includes E.coli Poly(A) Polymerase, RTase and RNasin. The E. coli Poly(A) Polymerase not only has high A-tailing efficiency, but also can specifically recognize single-strand miRNA, thus avoiding further reverse transcription reaction of miRNA precursors with double-strand structures. The RTase has been molecular modified to remove RNase H activity and increase the affinity of RNA template, thus enabling miRNA reverse transcription reaction to have higher efficiency and sensitivity. The 2× miRNA RT Reaction Buffer in this product contains all the raw materials and primers for miRNA A taling reaction and reverse transcription reaction. It is carefully optimized to ensure that the Poly(A) modification process and reverse transcription process at the 3' end of miRNA can be efficiently carried out at the same time.

  • Applications

    Fluorescence quantitative study of miRNA by dye-based/probe based method.

  • Kit Contents

  • Storage Condition

    Store at -30~-15°C for one year.

  • Important Notes

    This kit must be used together with miRcute Plus miRNA qPCR Kit (SYBR Green) (4992887/4992779).

  • Experimental Example

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