The miRcute Plus miRNA cDNA First-Strand cDNA Kit adopts the A-tailing method to carry out reverse transcription of miRNA first strand cDNA. The kit adopts a two-component form, thus simplifying the experimental operation process and reducing the possibility of operation errors. miRNA RT Enzyme Mix in this product includes E.coli Poly(A) Polymerase, RTase and RNasin. The E. coli Poly(A) Polymerase not only has high A-tailing efficiency, but also can specifically recognize single-strand miRNA, thus avoiding further reverse transcription reaction of miRNA precursors with double-strand structures. The RTase has been molecular modified to remove RNase H activity and increase the affinity of RNA template, thus enabling miRNA reverse transcription reaction to have higher efficiency and sensitivity. The 2×miRNA RT Reaction Buffer in this product contains all the raw materials and primers for miRNA A taling reaction and reverse transcription reaction. It is carefully optimized to ensure that the Poly(A) modification process and reverse transcription process at the 3’ end of miRNA can be efficiently carried out at the same time.