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Single Column

DNAsecure Plant Kit

For fast genomic DNA purification from various fresh plant tissues

Catalog number / packaging

Cat. no

ID

No. of preps

4992708

 DP320-03 200

4992707

 DP320-02 50
Manual Inquire Inspection report

  • Storage

    Room temperature (15-25℃)

  • Description

    DNAsecure Plant Kit adopts a high efficient nucleic acid-specific spin column and a unique buffer system to extract genomic DNA from a variety of fresh or cryopreserved plant tissues with minimal impurities remained. This kit does not require phenol or chloroform extraction and is safe to operate. Large fragments of genomic DNA with high purity and stable quality can be extracted.

  • Required Reagents

    Liquid nitrogen, ethanol

  • Features

    •    Simple and fast: The purified gDNA could be obtained within 1 hour.

    •    Non-toxic: Safe operation because no need of phenol or chloroform for extraction.

    •    High purity, excellent quality: 3-30 μg gDNA can be extracted from 100 mg plant tissues with the OD260/OD280 value at the range of 1.7-1.9.

  • Applications

    The DNAsecure Plant Kit can be used to purify DNA with the size up to 40kb. It is suitable for the following applications:

    •    PCR, qPCR and multiplex PCR

    •    DNA profiling techniques such as RAPD, RFLP, AFLP, SSR

    •    Library construction, Southern blot


  • DNA Extraction Yield From Various Plant Tissues

Experimental Example

  • Genomic DNA isolated from 100 mg leaves of different plants using DNAsecure Plant Kit. 3 μl of 100 μl eluates were loaded per lane.

    1: Fresh soybean leaves;

    2: Fresh tobacco leaves;

    3: Fresh wheat leaves;

    4: Fresh corn leaves.

    The concentration of the agarose gel was 1%. The electrophoresis was performed under 6 V/cm for 20 min.

    M: λDNA/Hind III Marker

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