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Single Column

TIANamp Soil DNA Kit

Rapid extraction of genomic DNA from various soil samples

Catalog number / packaging

Cat. no

ID

No. of preps

4992288

 DP336-02 50
Manual Inquire Inspection report

  • Storage

    Room temperature (15-25℃)

  • Description

    The TIANamp Soil DNA Kit uses a unique buffer system, by which the humic acid in soil sample could be completely removed. Zirconium beads are also applied in this kit to process the lysis of components of soil samples in order to guarantee the integrality of gDNA.

  • Required Reagents

    Ethanol

  • Features

    ■ Wide application: Isolate pure DNA from various soil samples, such as flower bed soil, potting soil, farmland soil, forest soil, sludge, red soil, black soil, dust and many other kinds of soil samples.

    ■ Rapid protocol: The whole experimental procedure could be finished in short time.

    ■ High purity: The application of spin column can ensure the high purity of DNA, which can be directly used in downstream experiments.

  • Applications

    The DNA extracted by this kit contain minimal impurities and have high integrity, which can be directly used in downstream molecular biology experiments such as PCR and restriction digestion.

  • Important Notes

    Excessive DNA may inhibit the downstream PCR reaction. In this case, it is recommended to dilute the DNA template before using.

Experimental Example

  • Genomic DNA purified from different soil samples by TIANamp Soil DNA Kit. Sample amount: 250 mg; 5 μl of 50 μl eluents were loaded per lane on 1% agarose gel.

    1. Black soil from Hei Long Jiang;  2. Laterite from Guangdong;

    3. Loess from Beijing;                   4. Laterite from Zhejiang;

    5. Laterite from Yunnan;                6. Laboratory dust;

    7. Forest soil;                                 8. Sludge;

    9. Farmland soil;                            10. Potting soil;

    11. Flower bed soil

  • Genomic DNA purified from different soil samples using TIANamp soil DNA kit were tested by PCR amplification. 6 μl of 20 μl PCR products were loaded per lane.

    1. Black soil from Hei Long Jiang;         2. Laterite from Guangdong;

    3. Loess from Beijing;                           4. Laterite from Guangxi;

    5. Laboratory dust;                               6. Forest soil;

    7. Potting soil;                                      8. Flower bed soil;

    9. Laterite from Zhejiang;                     10. Sludge;

    11. Farmland soil;

    M: TIANGEN Marker III

    NTC: negative control without templates

Publication

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