RNAprep Pure Tissue Kit provides a fast, simple, and cost-effective method for purification of total RNA from animal tissue, cultured cell and bacteria. The purified RNA is ready for use in downstream applications such as RT-PCR, real-time RT-PCR, gene-chips assay, northern blot, dot blot, polyA screening, in vitro transcript, molecular cloning and other downstream applications.

Note: GDP431H, GRT411 are shipped and packaged separately.

Lysozyme (self provide, TIANGEN, Cat. no GRT401-11, for bacteria RNA purification)

RNase-free DNase I, Buffer RDD and RNase-free ddH₂O (Tubular) should be stored at 2-8°C for 15 months; Buffer RL/β-mercaptoethanol mix can be stored at 2-8°C for 1 month; others stored at room temperature (15-30°C) for 15 months.

● β-Mercaptoethanol (β-ME) must be added to Buffer RL before use. The final concentration of β-ME is 1%. For example, add 10 μl β-ME to 1 ml Buffer RL. Buffer RL containing β-ME can be stored at 2-8°C for 1 month. Buffer RL may form a precipitate upon storage. If necessary, redissolve by warming, and then place at room temperature (15–30°C).

● Buffer RW is supplied as a concentrate. Before using for the first time, add ethanol (96–100%) as indicated on the bottle to obtain a working solution.

● Perform all steps in RT if not showed.

● Do not recommend storage sample in buffer with lysing capacity.

● Change gloves regularly. Bacteria on the skin can result in RNase contamination.

● Use RNase-free plastic and tips to avoid cross-contamination.

● RNA must be stored or applied in RNase-free plastic or glassware. To wipe off RNase, the glassware can be roasted at 150°C for 4 hours, while plastic can be dipped in 0.5 M NaOH for 10 min, washed by RNA-free ddH₂O thoroughly and sterilized.

● Use RNase-free ddH₂O to confect solution.