● High quality: Total RNA with high yield, high purity and good integrity can be extracted within 1 hour.

● Indicator included: Special indicator is added to make the lower layer pink after centrifugation, making the supernatant easy to be absorbed.

● High sensitivity: It has higher extraction efficiency for the extraction of trace samples such as viruses.

● Flexible sample amounts: Can be used for small amount of samples (50-100 mg tissue, 5x10⁶ cells), as well as for large amount of samples (≥1 g tissue or ≥10⁷ cells).

The TRNzol Universal Reagent is a newly developed total RNA extraction reagent containing an indicator based on TRNzol Reagent. The product has stronger lysis ability and higher sensitivity, and can extract total RNA from viruses, bacteria, fungi, animal and plant cells, tissues, body fluids etc. TRNzol Universal can fully lyse the sample, dissolve the cell contents, and effectively inhibit RNase activity, therefore efficiently extracting total RNA from the sample, and ensure the integrity of the RNA during extraction. The reaction can be completed in one hour.

Chloroform, isopropanol, RNase-Free ddH₂O, 75% ethanol (formulated with RNase-Free ddH₂O)

The table below presents typical yields of RNA from various starting materials.

Keep at 2-8°C away from light.

WARNING: This reagent is corrosive and should not be in direct contact with skin or be swallowed. Wear lab coats, masks, goggles and gloves when handling. If there is contact with skin, rinse immediately with detergent and plenty of water. This reagent is flammable.

● Before the addition of chloroform, homogenized samples in TRNzol Universal Reagent can be stored at -70°C for at least one month.

● The RNA can be stored in 75% ethanol for at least 1 year at -20°C, or at least 1 week at 2-8°C.

Method: 30 mg rat liver tissue, 100 mg rice leaves were collected by liquid nitrogen grinding; 1×10⁶ HepG2 cultured cells and 700 μl Saccharomyces Cerevisiae culture medium (OD₆₀₀ = 0.9) were collected by centrifugation. 1 ml of TRNzol Universal Reagent from TIANGEN and the relevant products from supplier L and T were added to each aliquot of sample and RNA extraction was performed following the protocols provided by each suppliers. The elution volume was 80 μl, 50 μl, 30 μl and 30 μl for the four samples respectively. 3 μl of the eluate was loaded per lane.

MIII: TIANGEN Marker III;

The electrophoresis was conducted at 6 V/cm for 30 min on a 1% agarose.

Results: TIANGEN TRNzol Universal Reagent can extract high purity and good integrity RNA from rat liver, rice leaves, cultured cells and yeast samples, with high efficiency. The RNA quality is comparable to or slightly higher than that of supplier L and T products.

● Change gloves regularly. Bacteria on the skin can result in RNase contamination.

● Use RNase-Free plastic and tips to avoid cross contamination.

● RNA can be protected in TRNzol Universal Reagent. But RNA must be stored or processed in RNase-Free plastic or glassware. To wipe off RNase, the glassware can be heated at 150°C for 4 hours, while plastic can be dipped in 0.5 M NaOH for 10 min, washed by RNase-Free ddH₂O thoroughly, and sterilized.

● Use RNase-Free ddH₂O to confect solution. (Add DEPC into water in clean glass container to a final concentration of 0.1% (v/v). Incubate overnight and autoclave for 15 min to remove any trace of DEPC.)