Precipitation Method

TRNzol Universal Reagent

New upgrade formula for wider sample adaptability

Catalog number / packaging

Cat. no

ID

No. of preps

4992730

DP424 100 ml
  • Packing Size

    100 ml
  • Storage

    Keep at 2-8℃ away from light

    WARNING: This reagent is corrosive and should not be in direct contact with skin or be swallowed. Wear lab coats, masks, goggles and gloves when handling. If contact with skin, rinse immediately with detergent and plenty of water. This reagent is flammable.

  • Description

    The TRNzol Universal Reagent is a newly developed total RNA extraction reagent containing an indicator based on TRNzol Reagent. The product has stronger lysis ability and higher sensitivity, and can extract total RNA from viruses, bacteria, fungi, animal and plant cells, tissues, body fluids etc. TRNzol Universal can fully lyse the sample, dissolve the cell contents, and effectively inhibit RNase activity, therefore efficiently extract total RNA from the sample, and ensure the integrity of the RNA during extraction. The reaction can be completed in one hour.

    The TRNzol Universal Reagent is a newly developed total RNA extraction reagent containing an indicator based on TRNzol Reagent. The product has stronger lysis ability and higher sensitivity, and can extract total RNA from viruses, bacteria, fungi, animal and plant cells, tissues, body fluids etc. TRNzol Universal can fully lyse the sample, dissolve the cell contents, and effectively inhibit RNase activity, therefore efficiently extract total RNA from the sample, and ensure the integrity of the RNA during extraction. The reaction can be completed in one hour.

  • Required Reagents

    Chloroform, isopropanol, RNase-Free ddH2O, 75% ethanol (formulated with RNase-Free ddH2O)
  • Features

    ■ High quality: Total RNA with high yield, high purity and good integrity can be extracted within 1 hour.

    ■ Indicator included: Special indicator is added to make the lower layer pink after centrifugation, making the supernatant easy to be absorbed.

    ■ High sensitivity: It has higher extraction efficiency for the extraction of trace samples such as viruses.

    ■ Flexible sample amounts: Can be used for small amount of samples (50-100 mg tissue, 5x106 cells), as well as for large amount of samples (≥1 g tissue or ≥107 cells).

  • Applications

    TRNzol Universal reagent minimizes contamination of impurities such as DNA and proteins in the purified total RNA, and can be directly used for various molecular biology experiments such as Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis, cDNA library construction, RT-PCR, Realtime PCR and high-throughput sequencing.

Experimental Example

  • Method: 30 mg rat liver tissue, 100 mg rice leaves were collected by liquid nitrogen grinding; 1×106 HepG2 cultured cells and 700 μl Saccharomyces Cerevisiae culture medium (OD600=0.9) were collected by centrifugation. 1 ml of TRNzol Universal Reagent from TIANGEN and the relevant products from supplier L and T were added to each aliquot of sample and RNA extraction was performed following the protocols provided by each suppliers. The elution volume was 80 μl, 50 μl, 30 μl and 30 μl for the four samples respectively. 3 μl of the eluate was loaded per lane.

    MIII: TIANGEN Marker III;

    The electrophoresis was conducted at 6 V/cm for 30 min on 1% agarose gel.

    Results: TIANGEN TRNzol Universal Reagent can extract high purity and good integrity RNA from rat liver, rice leaves, cultured cells and yeast samples, with high efficiency. The RNA quality is comparable to or slightly higher than that of supplier L and T products.

  • Method: 30 mg rat liver tissue, 100 mg rice leaves were collected by liquid nitrogen grinding; 1×106 HepG2 cultured cells and 700 μl Saccharomyces Cerevisiae culture medium (OD600=0.9) were collected by centrifugation. 1 ml of TRNzol Universal Reagent from TIANGEN and the relevant products from supplier L and T were added to each aliquot of sample and RNA extraction was performed following the protocols provided by each suppliers. The elution volume was 80 μl, 50 μl, 30 μl and 30 μl for the four samples respectively. 3 μl of the eluate was loaded per lane.

    MIII: TIANGEN Marker III;

    The electrophoresis was conducted at 6 V/cm for 30 min on 1% agarose gel.

    Results: TIANGEN TRNzol Universal Reagent can extract high purity and good integrity RNA from rat liver, rice leaves, cultured cells and yeast samples, with high efficiency. The RNA quality is comparable to or slightly higher than that of supplier L and T products.

  • Method: 30 mg rat liver tissue, 100 mg rice leaves were collected by liquid nitrogen grinding; 1×106 HepG2 cultured cells and 700 μl Saccharomyces Cerevisiae culture medium (OD600=0.9) were collected by centrifugation. 1 ml of TRNzol Universal Reagent from TIANGEN and the relevant products from supplier L and T were added to each aliquot of sample and RNA extraction was performed following the protocols provided by each suppliers. The elution volume was 80 μl, 50 μl, 30 μl and 30 μl for the four samples respectively. 3 μl of the eluate was loaded per lane.

    MIII: TIANGEN Marker III;

    The electrophoresis was conducted at 6 V/cm for 30 min on 1% agarose gel.

    Results: TIANGEN TRNzol Universal Reagent can extract high purity and good integrity RNA from rat liver, rice leaves, cultured cells and yeast samples, with high efficiency. The RNA quality is comparable to or slightly higher than that of supplier L and T products.

  • Method: 30 mg rat liver tissue, 100 mg rice leaves were collected by liquid nitrogen grinding; 1×106 HepG2 cultured cells and 700 μl Saccharomyces Cerevisiae culture medium (OD600=0.9) were collected by centrifugation. 1 ml of TRNzol Universal Reagent from TIANGEN and the relevant products from supplier L and T were added to each aliquot of sample and RNA extraction was performed following the protocols provided by each suppliers. The elution volume was 80 μl, 50 μl, 30 μl and 30 μl for the four samples respectively. 3 μl of the eluate was loaded per lane.

    MIII: TIANGEN Marker III;

    The electrophoresis was conducted at 6 V/cm for 30 min on 1% agarose gel.

    Results: TIANGEN TRNzol Universal Reagent can extract high purity and good integrity RNA from rat liver, rice leaves, cultured cells and yeast samples, with high efficiency. The RNA quality is comparable to or slightly higher than that of supplier L and T products.