Column-based Method

RNAprep Pure Hi-Blood Kit

For purification of high-quality and stable total RNA from blood

Catalog number / packaging

Cat. no	ID	No. of preps
4992903	DP443	50

Manual Inquire

Storage

Buffer RLH with β-mercaptoethanol can be stored at 4℃ for one month. DNase I, Buffer RDD, should be stored at 2-8℃.

Other reagents could be store at room temperature (15-25℃)

Buffer RLH with β-mercaptoethanol can be stored at 4℃ for one month. DNase I, Buffer RDD, should be stored at 2-8℃.

Other reagents could be store at room temperature (15-25℃)
Description

The RNAprep Pure Hi-Blood Kit efficiently extracts total RNA from fresh whole blood and blood with multiple anticoagulants from different species. The silicon matrix material used in the adsorption column is a unique new material developed by TIANGEN, which efficiently and specifically adsorbs RNA, and removes impurity proteins to the utmost extent. The extracted RNA can be used in various downstream experiments such as RT-PCR, RT-qPCR, chip analysis, high-throughput sequencing, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis, molecular cloning, etc.

本キットは新鮮な全血からトータルRNAを効率的に抽出し、様々な種及び多種の抗凝固剤の全血サンプルを処理することができます。スピンカラムに採用したシリカメンブレンは当社特有の新材料であり、RNAを効率的かつ特異的に吸着し、不純物タンパク質を最大限に除去することができます。抽出されたRNAはRT-PCR、Real Time RT-PCR、マイクロアレイ、ハイスループット遺伝子配列決定、Northern Blot、Dot Blot、Poly Aスクリーニング、In vitro翻訳、RNase保護分析と分子クローンなど多くの下流のアプリケーションに使用できます。
Required Reagents

β-mercaptoethanol, ethanol

β-mercaptoethanol, ethanol
Features

■ Suitable for fresh whole blood of different species, easy to operate.

■ Equipped with RNase-Free Filtration Column CS to effectively remove impurities.

■ The specifically formulated buffer can ensure efficient and stable RNA extraction for a variety of downstream experiments.

■ Safe and reliable operation, no phenol/chloroform extraction required.

■異なる種の新鮮な全血に適用し、操作が簡単。

■Filtration Columns CSが付いているので、不純物を効果的に除去することができる。

■最適化されたバッファーは、RNA抽出の高効率と安全性を確保し、多様な下流のアプリケーションに適している。

■操作が安全で、フェノール/クロロホルム抽出が不要。
Application

RT-PCR, Northern Blot, RT-qPCR, chip analysis, high-throughput sequencing, PolyA screening, RNase protection analysis, in vitro translation, etc.

RT-PCR, Northern Blot, RT-qPCR, chip analysis, high-throughput sequencing, PolyA screening, RNase protection analysis, in vitro translation, etc.

Figure 1. RNA purified from 100 μl fresh rat blood in different anticoagulants using RNAprep Pure Hi-Blood Kit. 4-6 μl of 50 μl eluates were loaded per lane.

M: TIANGEN DNA Marker III.

Figure 1. RNA purified from 100 μl fresh rat blood in different anticoagulants using RNAprep Pure Hi-Blood Kit. 4-6 μl of 50 μl eluates were loaded per lane.

M: TIANGEN DNA Marker III.
Figure 2. RNA purified from 100 μl fresh mouse blood using RNAprep Pure Hi-Blood Kit. 4-6 μl of 50 μl eluates were loaded per lane.

M: TIANGEN DNA Marker III.

Figure 2. RNA purified from 100 μl fresh mouse blood using RNAprep Pure Hi-Blood Kit. 4-6 μl of 50 μl eluates were loaded per lane.

M: TIANGEN DNA Marker III.

Column-based Method

RNAprep Pure Hi-Blood Kit

Catalog number / packaging

Storage

Description

Required Reagents

Features

Application

Publication