Column-based Method

RNAprep Pure Hi-Blood Kit

For purification of high-quality and stable total RNA from blood

Catalog number / packaging

Mat. No

Ref. No

No. of preps

4992903

GDP443 50
  • Features

    ● Suitable for fresh whole blood of diferent species, easy to operate.

    ● Equipped with RNase-Free Filtration Column CS to effectively remove impurities.

    ● The specifically formulated buffer can ensure efficient and stable RNA extraction for a variety of downstream experiments.

    ● Safe and reliable operation, no phenol/chloroform extraction required.

  • Description

    The RNAprep Pure Hi-Blood Kit efficiently extracts total RNA from fresh whole blood and blood with multiple anticoagulants from diferent species. The silicon matrix material used in the adsorption column is a unique new material developed by TIANGEN, which efficiently and specifically adsorbs RNA, and removes impurity proteins to the utmost extent. The extracted RNA can be used in various downstream experiments such as RT-PCR, qRT-RCR, chip analysis, high-throughput sequencing, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis, molecular cloning, etc.
  • Kit Contents

  • Application

    RT-PCR, Northern Blot, qRT-PCR, chip analysis, high-throughput sequencing, PolyA screening, RNase protection analysis, in vitro translation, etc.
  • Required Reagents

    β-mercaptoethanol, ethanol
  • Storage Condition

    Buffer RLH with β-mercaptoethanol can be stored at 4℃ for one month. DNase I, Bufer RDD, should be stored at 2-8℃.

    Other reagents could be store at room temperature (15-30℃).

  • Important Notes

    ● Before operation, add β-mercaptoethanol to Buffer RLH to a final concentration of 1%. (For example, add 10 μl β- mercaptoethanol to 1 ml RLH). It’s better to prepare the buffer when using. The prepared RLH can be stored at 2-8°C for one month. The precipitate might formed in Buffer RLH during storage.If there is precipitate, please use it after heating and dissolving.

    ● Add 96-100% ethanol to Buffer RW and Buffer RW1H before the first time using. Please refer to the label on the bottle for the added volume.

    ● Human blood or body fluid may have potential infectivity, so please pay attention to take protective measures when processing human whole blood.

    ● The kit can process up to 1.5 ml of healthy human whole blood (the leukocyte content in the whole blood of healthy people is up to 4000-7000 cells/μl blood). If the leukocyte content in the blood is high, the amount of blood can be reduced proportionally, and the maximum number of leukocyte that can be processed by the kit is 1×107

    ● After leukocyte lysis, all the steps in this manual should be carried out at room temperature (15-30°C), and please perform all steps as fast as possible.

    ● Cell lysate (in Buffer RLH) can be stored at -70°C When ready to use, incubate it at 37°C for 10 minutes to ensure that all salts are dissolved, then proceed to step 7 of the procedure.

    ● This kit is not suitable for cryopreserved whole blood.

  • Experimental Example

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