The TIANquick Midi Purification Kit adopts a unique adsorption column to efficiently and specifically bind DNA fragments while maximally removing impurities such as proteins, ions and small primers. This kit can be used for PCR product recovery as well as enzymatic reaction solution recovery (enzymatic digestion, ligation, probe labeling, etc.). 100 bp-20 kb DNA fragments can be recovered with the recovery rate up to 80% (for DNA fragments< 100 bp or > 10 kb, the recovery rate is 30%-50%). The purified DNA can be directly used for molecular biology experiments such as ligation, transformation, restriction enzyme digestion, sequencing, hybridization, etc.