Pfu DNA Polymerase is expressed from E.coli with cloned Pyrococus Furiosis DNA Polymerase gene and purified and separated by multiple column purification. Because Pfu has 3'-5' exonuclease activity, it can proofread in DNA amplification process, while traditional Taq DNA Polymerase cannot. Although other Taq DNA polymerases such as Vent, Deep Vent, Tli, UITma, etc. have proofreading functions, Pfu has the lowest mismatch rate among all Taq DNA polymerases found so far. Pfu DNA Polymerase has better thermal stability than common Taq DNA polymerase, and it can maintain more than 90% activity at 95°C for 1 hour.

One-tube Pfu PCR Mix (China National High-Tech Product Certification)

■ The Pfu PCR Mix has improved specificity and sensitivity of PCR reaction and can amplify complex templates with high GC content, secondary structure and the like. As low as 2 copies of the target template can be amplified, ensuring more accurate experimental results.

■ The unique Pfu MasterMix formula makes the whole reaction system very stable, and the activity will not be affected by repeated freeze-thaw or long-term storage at 4°C.

■ The stable and efficient pre-prepared PCR mix solution can make the operation fast and simple, greatly reducing labor intensity and sampling error. High-performance PCR enhancer and optimizer are also included in the mix, which reduces the requirements on PCR conditions.

■ This product has both dye-containing and dye-free systems. Dye-containing PCR Mix products can be directly electrophoresed after PCR, without adding sample buffer.

50 mM Tris-HCl (pH8.2)

0.1 mM EDTA

1 mM DTT

50% Glycerol

Stabilizers