The FastKing One Step RT-PCR Kit adopts the one-step method to enable RT and PCR to be carried out in the same reaction system. No reagent needs to be added and the tube cover does not need to be opened in the reaction process, thus avoiding cross contamination between samples and improving the detection sensitivity. The 25×RT-PCR Enzyme Mix in the kit is a premixed mix form of TIANGEN novel reverse transcriptase (King RTase), antibody modified hot-start Taq DNA polymerase and RNase Inhibitor. It has stronger RNA affinity and thermal stability, extension capability to complex secondary structure RNA templates, and higher amplification efficiency and specificity to cDNA after reverse transcription. In addition, the 2× FastKing One Step RT-PCR MasterMix in this product is a new type of reaction system specially optimized for the above two key enzymes, which contains necessary ionic components, dNTPs, PCR stabilizers and enhancers. It ensures that King RTase and Taq polymerases have the best performance in the whole one-step reaction process.