FastReal qPCR PreMix (SYBR Green) can be stored at -30~-15°C for one year.

It should be stored immediately upon receipt at -30~-15°C. 2 × FastReal qPCR PreMix (SYBR Green I) and 50 × ROX Reference Dye should be thawed and then mixed upside down gently to be homogenous before using. If the reagents have been thawed but not used, it is important to thoroughly mix prior to re-freezing. The layering of salts during the thawing process and subsequent crystallization during freezing will damage the enzyme and decrease product performance. 

The reagents could be stored for up to 3 months at 2-8°C if frequently used. Please avoid refreezing and thawing repeatedly.

FastReal qPCR PreMix (SYBR Green) is designed for SYBR Green I based quantitative PCR assays, enables highly sensitive, rapid and specific quantitative detection of target DNA.

1.Fast reaction. The hot-start Taq DNA polymerase in the FastReal qPCR PreMix has short hot-start time, high enzyme activity and fast reaction; the components in the reaction buffer, after targeted modification, can greatly shorten the denaturation, annealing and extension time, which can save up to 50% of the reaction time and obtain the experimental results quickly.

2. High amplification capacity. The new hot-start Taq DNA polymerase, combined with the optimized Fast PCR buffer, ensures high amplification efficiency and product specificity of the reagent, and features early peak onset and high fluorescence value of the amplification curve.

3. Transparent tube, reagent residual is clearly visible. The 2 × FastReal qPCR PreMix is packaged in colorless transparent tubes with good light stability, and the transparent tubes are more convenient for customers to access.

4. The instrument is widely applicable. It is not only suitable for fast quantification instruments, but also for common quantification instruments.

FastReal qPCR PreMix (SYBR Green) is designed for SYBR Green I based quantitative PCR assays, enables highly sensitive, rapid and specific quantitative detection of target DNA. Optimized premix could reduce the running time and is suitable for regular and fast real-time PCR thermal cycler. The hot-start Taq DNA polymerase used in the FastReal qPCR PreMix is modified with a novel antibody. Combined with the optimized Fast PCR buffer, it could ensure a sensitive PCR detection on any Real-Time PCR thermal cycler. It features fast reaction, high amplification efficiency, high amplification specificity, high sensitivity, early peak amplification curve and high fluorescence value, allowing you to obtain results faster and save research time and energy without compromising PCR results.