● Simple and fast: Automated extraction allows for easy access to higher purity RNA in a short time.

● Safe and non-toxic: No toxic reagents such as phenol/chloroform.

● High purity: The RNA obtained is of high purity and can be directly used for microarray detection, high-throughput sequencing and other experiments.

The kit adopts magnetic beads and a unique buffer system, to isolate and purify total RNA with high quality from cell/tissue. The uniquely embedded magnetic beads have a strong affnity for nucleic acid under certain conditions. When the conditions are changed, the magnetic beads can release the absorbed nucleic acid to rapidly separate and purify it.

It can be used to perfectly fit with automated nucleic acid extractor. Through absorption, transfer and release of magnetic beads by the special magnetic bar, magnetic beads and nucleic acid can be transferred to improve the degree of automation. The whole process is safe and convenient, and the extracted RNA has high purity. If high throughput automated extraction is required, TIANGEN can deliver integrated solutions.

RNA purified by this kit is suitable for downstream experiments including RT-PCR, Real Time RT-PCR, chip analysis, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis and molecular cloning.

Note: GDP671-DH, GRT431 and OSE-TGA-S36 are shipped and stored separately.

● To use the TGuide Smart Universal RNA Kit, you must have the TGuide Smart Universal RNA (program no. DP671) installed on the TGuide S16/S32 pro Nucleic Acid Extractor.

● This product is suitable for TGuide S16/S32/S32 Pro/S96 Dex fully automated nucleic acid extractor.

● Wear a mask during the experiment and change new gloves frequently to avoid RNase contamination.

● Avoid cross-contamination by using RNase-free plastics and tips.

● Pay attention to the optimal storage and pretreatment conditions of the samples to avoid causing degradation of the extracted RNA.

Dissolve DNase I dry powder (1500 U) in 1 ml RNase-Free ddH2O, mix gently and follow the protocol to add 10 μl to each sample for freshly use. 

Attention: If long-term storage needed, dissolve DNase I dry powder (1500 U) in 550 μl RNase-Free ddH2O to make stock solution, mix gently, dispense and store at -30~-15°C (can be stored for 9 months). Add 5 μl stock solution to each sample. Store the DNase I stock solution after thawing from -30~-15°C at 2-8°C (can be stored for 6 weeks) and do not freeze it again.