Smart Series Extraction Kits
InquireCatalog Number｜Packaging
Mat. No |
Ref. No |
No. of preps |
|
4996194 |
GDP670-DE | 48（ Single Cartridge） |
|
4996181 |
GDP670-E | 96（Plate） |
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Description
TGuide Smart Micro Cell Total RNA Kit is designed for rapid and efficient isolation of high-quality total RNA from low cell number samples. Using advanced magnetic bead technology and optimized buffer chemistry, the kit ensures reliable RNA purification with high yield and purity.
Compatible with TGuide S16, S32, S32 Pro, and S96 Dex automated nucleic acid extraction systems, this kit enables safe, convenient, and automated RNA extraction for molecular biology research and downstream applications.
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Features
- Simple and Fast: Combined with TGuide S16/S32/S32 Pro/S96 Dex automated extraction systems, high-quality RNA can be obtained in a short time.
- Safe and Non-Toxic: No toxic reagents such as phenol or chloroform are required.
- High Purity: High-purity RNA can be obtained from low numbers of cells for downstream experiments.
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Applications
The purified RNA is suitable for various downstream molecular biology applications, including:
- RT-PCR
- Real-Time RT-PCR (RT-qPCR)
- Microarray Analysis
- Northern Blot
- Dot Blot
- Poly(A) Selection
- In Vitro Translation
- RNase Protection Assays
- Molecular Cloning
Experimental Example
Sample Description: Cryopreserved cell samples and fresh cell samples were cultured from the same batch. During extraction of the fresh cell samples, the same lysis buffer was added to the cryopreserved samples, which were then stored at -80°C for 3 days. Before extraction, the frozen samples were thawed directly and processed. A total of 13 parallel samples were extracted using TIANGEN and Brand A, respectively.
Sample Type and Quantity: 4 × 104 primary rat hepatocytes.
Conclusion: The concentrations of total RNA extracted using DP670 and a comparable commercial product were essentially equivalent. Quantification results for the Actin and GAPDH genes demonstrated that DP670 delivered performance comparable to that of the commercial counterpart.
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