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Features
● Easy and fast: Ultrapure genomic DNA can be obtained within 48 min. Widely applicable: Applicable to all kinds of animal tissues.
● Ultrapure: The obtained DNA has high purity and can be directly used in molecular biology experiments such as PCR, enzyme digestion, hybridization, etc.
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Description
The kit adopts magnetic beads with unique separation function and a unique buffer system to separate and purify high-quality genomic DNA from various animal tissues. The unique embedded magnetic beads have strong affinity for nucleic acid under certain conditions, and when the conditions change, the magnetic beads release adsorbed nucleic acid, thus achieving the purpose of fast separation and purification of nucleic acid.
The product is perfectly matched with TGuide S32 Automated Nucleic Acid Extractor. Magnetic beads are adsorbed, transferred and released by special magnetic rods, thus realizing the transfer of magnetic beads and nucleic acids. The whole experimental process is safe and convenient. The extracted genomic DNA fragments are large in size, with high purity, stable and reliable quality.
The DNA purified by the kit is suitable for various conventional operations, including enzyme digestion, PCR, library construction, Southern blot and other experiments.
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Kit Contents
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Applications
● The product is perfectly matched with TGuide S32 Automated Nucleic Acid Extractor to achieving the purpose of fast separation and purification of nucleic acid
● The DNA purified by the kit is suitable for various conventional operations, including enzyme digestion, PCR, library construction, Southern blot and other experiments
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Storage Condition
This kit can be stored at room temperature (15-30°C) under dry condition for 12 months. If a precipitate has formed in Buffer under 2-8°C, please place the buffer at room temperature or warm at 37°C for 10 min to dissolve the precipitate.
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Detection of DNA concentration and purity
The size of the obtained genomic DNA fragment is related to factors such as sample storage time and shearing force during operation. The concentration and purity of the obtained DNA fragments can be detected by agarose gel electrophoresis and ultraviolet spectrophotometer.
DNA should have a significant absorption peak at OD260, with OD260 value of 1 equivalent to about 50 μg/ml double stranded DNA and 40 μg/ml single stranded DNA.
The ratio of OD260/OD280 should be 1.7-1.9. If deionized water is used instead of elution buffer, the ratio will be low, because the pH value and the presence of ions will affect the light absorption value, but it does not mean the purity is low.
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