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Features
● The unique magnetic rod vibration mode has large vibration amplitude and high precision.
● New strong binding mode, good magnetic beads binding effect and high nucleic acid yield.
● Limit position protection function, stable and orderly operation of the instrument to avoid failure.
● Windows Pad and screen button double control mode, powerful program editing function, intuitive, simple and convenient operation.
● Smart contamination control system and UV sterilization module can effectively avoid cross contamination between sample wells and batches.
● Relying on TIANGEN nucleic acid extraction and purification reagent platform, it provides a series of comprehensive, complete and high-quality automation solutions.
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Description
TGuide S32 Automated Nucleic Acid Extractor adopts the latest magnetic rods binding and transfer method. 96-deep well plates and different kinds of magnetic beads extraction reagents are applied to automatically extract and purify nucleic acids from 1-32 blood/cells/ tissues/viruses and other samples. The instrument uses the special magnetic rods to bind, transfer and release magnetic beads, so as to realize the automatic transfer of magnetic beads and nucleic acids.
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Parameters
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Principle
After the sample is lysed by the lysis buffer, the nucleic acid dissociated from the lysis/binding solution is specifically bond by the magnetic beads. Through the cooperation of the magnetic rod and the magnetic tip comb, the magnetic binding, transfer, release, mixing and other actions are completed to separate the magnetic beads binding the nucleic acid from the lysis/binding solution. Various impurities that are not specifically bond to the magnetic beads are removed in the washing well, and finally the nucleic acid molecules are dissolved in the elution buffer.
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Required Reagents
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Unique Movement Mode of Magnetic Rod
The driving device adopts a stepping motor. With large vibration amplitude of the magnetic rod, the vibration amplitude is automatically adjusted according to the volume of the solution, making sure a good mixing effect. The transmission device adopts a ball screw, leading to stable operation of magnetic rod, high precision and long service life. All moving parts are equipped with limit position protection function to ensure smooth and orderly operation of the instrument to avoid failures.
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New Strong Adsorption Mode
Through a newly designed strong binding mode, magnetic beads are bond on the tip of the magnetic rod, so that under the condition of small elution volume, eluent can still cover all magnetic beads. The magnetic beads have good binding effect and high nucleic acid yield.
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Dual Control Mode of Windows Pad and Screen Buttons
On the basis of the classic button operation, the TGuide S32 can also be remotely controlled with high-performance Windows Pad. The interactive interface of Windows application program is perfectly set, which is more in line with customers’ normal office working and learning habits.
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Experimental Example
Blood Genomic DNA Extraction:
Virus DNA/RNA Extraction:
Animal Tissues Genomic DNA Extraction:
Mouth Swabs Genomic DNA Extraction:
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