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Features
● Simple and fast: High-quality viral DNA/RNA can be obtained by running TGuide S16 within half an hour.
● Flexible throughput: It is a perfect fit for TGuide S16 Nucleic Acid Extractor to extract 1~8 samples.
● Wide use: It is applicable to various cell-free body fluid samples, blood and tissue suspensions.
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Description
The kit adopts magnetic beads and a unique buffer system, to isolate and purify viral DNA/RNA with high quality from blood, tissue, serum, plasma, lymph, cell-free body fluid, cell culture supernatant, urine, or various viral preservation solutions. The uniquely embedded magnetic beads have a strong affinity for nucleic acid under certain conditions. When the conditions are changed, the magnetic beads can release the absorbed nucleic acid to rapidly separate and purify it. The extracted viral DNA/RNA has high yield, high purity, stable and reliable quality. It can perfectly fit with TGuide S16 Nucleic Acid Extractor of TIANGEN for automated extraction. Nucleic acid purified by this kit is suitable for downstream experiments including RT-PCR, PCR, and qPCR. -
Kit Contents
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Applications
For purification viral DNA/RNA from blood, tissue, serum, plasma, body fluid, swab, tissue sputum, etc. -
Storage Condition
The kit can be stored in dry conditions at room temperature (15~30°C) for 12 months. -
Important Notes
● To use the TGuide Smart Viral DNA/RNA Kit, you must have the TGuide Smart Viral DNA/RNA (program no. DP604-01) installed on the TGuide S16/S32 pro Nucleic Acid Extractor.
● The sample should be avoided repeated freeze-thaw, otherwise it will lead to reduced nucleic acid extraction. The sample can be extracted immediately or stored at 4°C for less than 24 hr. For long-term storage, it can be stored at -20°C or -80°C.
● When extracting viral DNA/RNA by the kit, special attention should be paid to prevent nucleic acid degradation. The utensils, sample injectors, etc. used should be dedicated, and disposable consumables such as centrifuge tubes and pipette tips should go through autoclaved sterilization. Operators should wear powderfree gloves, masks, etc.
● There may be magnetic beads residual in the 5th well at elution step, so try to avoid touching magnetic beads during final sample transfer. If magnetic particles are present in user’s storage tube, centrifuge at 10,000 ×g for 2 minutes, and transfer the supernatant to a clean tube (not provided).
● The samples and reagent materials used should be disposed properly, and the operating table should be cleaned and disinfected thoroughly.
● It is necessary to take an appropriate amount of the processed sample and add it to 1st well of the single sample reagent cartridge.
● The elution buffer (RNase-Free ddH2O) is not prefilled, so you need to add the elution buffer (RNase-Free ddH2O) to the 5th well of the single sample reagent cartridge before performing on the instrument. The recommended range of elution buffer is 60~100 µl. The smaller the elution volume, the higher the nucleic acid concentration, but the lower the total yield may be. On the contrary, the larger the elution volume, the lower the nucleic acid concentration, and the higher the total yield may be. You can adjust it in the range of 60~100 µl as needed.
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Operational Steps
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