● Simple and fast: Ultra-pure genomic DNA can be obtained by running TGuide S16 for 50 minutes.

● Wide use: It is applicable to all kinds of animal tissue and cells.

● Ultra-pure: The obtained DNA has high purity and can be directly used in PCR, digestion, hybridization and other molecular biological experiments.

This kit adopts magnetic beads and a unique buffer system to separate and purify high-quality genomic DNA from various animal tissues and cells. The uniquely embedded magnetic beads have a strong affinity for nucleic acid under certain conditions. When the conditions are changed, the magnetic beads can release the absorbed nucleic acid to rapidly separate and purify the nucleic acid.

It can be used to perfectly fit with TGuide S16 Nucleic Acid Extractor. Through absorption, transfer and release of magnetic beads by the special magnetic bar, magnetic beads and nucleic acid can be transferred to improve the degree of automation. The whole process is safe and convenient, and the extracted genomic DNA fragments are large, with high purity and reliable quality.

The DNA purified by this kit is suitable for a range of common downstream applications including digestion, PCR, library construction, Southern hybridization, and other experiments.

For genomic DNA purification from tissue and cells. 

The kit can be stored under dry conditions at room temperature (15~30°C) for 12 months. If the solution precipitates, it can be preheated in a water bath at 37°C for 10 min to dissolve the precipitation, without affecting the effect.

● Repeated freezing and thawing samples should be avoided, otherwise the extracted DNA fragments will be small and the total yield will decrease.

● If there is precipitation in the Buffer GHA, it can be dissolved in a 37°C water bath and used after shaking well.

The size of the obtained genomic DNA fragment is affected by the sample storage time and shear force during operation. The concentration and purity can be detected by agarose gel electrophoresis and UV spectrophotometer. Ideally, the DNA should absorb at most at OD260, where an OD260 value of 1 corresponds to approximately 50 μg/ml double strand DNA and 40 μg/ml single strand DNA. The OD260/OD280 ratio should be 1.7~1.9.