Store at -20°C and avoid freezing and thawing many times. Aliquot the 10× T4 DNA Ligation Buffer to store, and take out appropriate amount when using.

T4 DNA Ligase catalyzes the formation of a phosphodiester linkage between 5'-phosphoryl group and adjacent 3'-hydroxyl group of duplex DNA or RNA, or DNA/RNA hybridization in a blunt end or cohesive end configuration. The enzyme can also catalyze the ligation of double stranded RNA and double stranded DNA, but can not catalyze the ligation of single stranded nucleic acid. ATP is required for the reaction.