miRNA Reagents
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Storage
Store in dark at -20°C -
Description
The kit adopts the principle of SYBR® Green I chimeric fluorescence to carry out miRNA fluorescence quantitative detection. The kit contains all reagents for quantitative miRNA fluorescence detection, including 2×miRcute Plus miRNA Premix, 50×ROX Reference Dye and Reverse Primer. Among them, 2× miRcute Plus miRNA Premix (With SYBR & ROX) is a new generation of premixed fluorescent quantitative PCR detection reagent specially developed for miRNA quantitative detection. The DNA Polymerase uses chemically modified hot-start polymerase, cooperating with a special buffer system, it has better reaction specificity, higher sensitivity, and accurate quantification can be carried out in a wider range. -
Features
■ High sensitivity: Target miRNA of total RNA of pg grade can be detected.
■ Strong specificity: The completely upgraded system can better distinguish single base differences and perfectly distinguish different miRNA within family.
■ Good versatility: Suitable for various types of real time PCR instruments.
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Applications
■ Detect reverse transcribed cDNA of miRNA by real time PCR -
Important Notes
■ This kit must be used together with miRcute Plus miRNA First-Strand cDNA Kit (4992786/4992909).
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