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Storage
Store at -20°C -
Description
The lnRcute lncRNA First-Strand cDNA Kit is a product specially developed for reverse transcription of long-chain non-coding RNA (lncRNA). Compared with mRNA, lncRNA has the characteristics of low abundance, large difference in GC content and more complex secondary structure, and traditional reverse transcription reagents are difficult to achieve ideal effects on lncRNA. This kit contains gDNase for efficiently removing genomic DNA, which can effectively avoid interference of residual genomic DNA on subsequent detection results. The reverse transcriptase used in lnR RT Enzyme Mix in this kit is FastKing RT Enzyme. This enzyme is a new molecular modified reverse transcriptase, with a hydrophobic motif added, and has stronger RNA affinity and thermal stability, which makes this kit outstanding in aspects of stress resistance and high reverse transcription efficiency for templates with GC content, complex secondary structure or low abundance. This kit is especially suitable for reverse transcription reaction of lncRNA with relatively low expression level and relatively complex secondary structure. -
Features
■ High efficiency: Stable and efficient King reverse transcriptase with RT efficiency over 95%.
■ Sensitivity: The sensitivity-enhancing components are added to the buffer solution, ensuring the high sensitivity with lower limit of detection reaching 0.1 ng total RNA.
■ Stress resistance: Suitable for complex templates, perfect resistance to foreign interference
■ Fast reaction: gDNA-free cDNA can be synthesized within 21 min
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Applications
■ The reverse transcribed cDNA can be used for real time PCR of lncRNA, and for regular PCR of mRNA, real time PCR, cDNA library construction and other experiments.
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