Menu
English

English

Janpanese(Japan)

Korean(Korea)

Methylation

DNA Bisulfite Conversion Kit

he conversion and purifi cation can be completed in 2 hour, with the conversion rate up to 99%

Catalog number / packaging

Cat. no

ID

No. of preps

4992447

 DP215-02 50
Manual Inquire Inspection report

  • Storage Conditions

    Store at room temperature (15-25°C).

  • Description

    This product is specially developed for DNA bisulfite conversion in DNA methylation research. The kit can finish the treatment of DNA samples in 2 hours, and the conversion rate of unmethylated cytosine to uracil is over 99%. This kit adopts a unique component of DNA protective agent, which guarantees the quality and recovery of DNA after conversion. In addition, this kit also uses the method of on-column sulphurous acid group removal, which makes the whole operation process easier.

  • Required Reagents

    Ethanol

  • Features

    ■ Simple and fast: The conversion and purifi cation process can be completed in 2 hours, with low requirements for instruments and equipment, which is suitable for research institutions at all levels.

    ■ High conversion rate: The conversion rate of unmethylated cytosine to uracil in DNA sample is over 99%.

    ■ High sensitivity: This kit can process DNA samples of as low as 500 pg and up to 2.5 μg.

  • Applications

    The DNA samples processed by this kit are suitable for methylation specifi c PCR/qPCR, sequencing and microarray.

  • Important Notes

    ■ If the treated DNA sample is less than 100 ng, it is strongly recommended that Carrier RNA be added to Buff er PB to a fi nal concentration of 10 μg/ml.

Experimental Example

  • Table 1: Conversion rate of unmethylated “C” to “U”

    Using human genome as experimental material, the recovery rate of the sample can reach 65%, and the ratio of

    unmethylated “C” to “U” can reach more than 99%.

Publication

Inquire