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Storage Conditions
Store at room temperature (15-25°C). -
Description
This product is specially developed for DNA bisulfite conversion in DNA methylation research. The kit can finish the treatment of DNA samples in 2 hours, and the conversion rate of unmethylated cytosine to uracil is over 99%. This kit adopts a unique component of DNA protective agent, which guarantees the quality and recovery of DNA after conversion. In addition, this kit also uses the method of on-column sulphurous acid group removal, which makes the whole operation process easier.
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Required Reagents
Ethanol
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Features
■ Simple and fast: The conversion and purifi cation process can be completed in 2 hours, with low requirements for instruments and equipment, which is suitable for research institutions at all levels.
■ High conversion rate: The conversion rate of unmethylated cytosine to uracil in DNA sample is over 99%.
■ High sensitivity: This kit can process DNA samples of as low as 500 pg and up to 2.5 μg.
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Applications
The DNA samples processed by this kit are suitable for methylation specifi c PCR/qPCR, sequencing and microarray.
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Important Notes
■ If the treated DNA sample is less than 100 ng, it is strongly recommended that Carrier RNA be added to Buff er PB to a fi nal concentration of 10 μg/ml.
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