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lncRNA Reagents

lnRcute lncRNA qPCR Kit (SYBR Green)

Sensitive and stress-resistant special fluorescent quantitative reagent for lncRNA

Catalog number / packaging

Cat. no

ID

No. of preps

4992886

 FP402-02 20 μl×500 rxn
Manual Inquire Inspection report

  • Storage

    Store at -20°C

  • Description

    Compared with mRNA, lncRNA has the characteristics of low abundance, large difference in GC content and more complex secondary structure, and traditional quantitative reagents are difficult to achieve ideal effects on lncRNA. The 2×lnR lncRNA PreMix in this kit is a new generation of pre-mixed real time PCR detection reagent specially developed for quantitative detection of lncRNA. The antibody modified hot-start DNA Polymerase ensures that the product has higher detection sensitivity while ensuring higher reaction specificity. In addition, the addition of H-competitor factor and EP component in Buffer makes the product have a wide range of sample universality. It has very good amplification applicability for templates with different GC contents, complex advanced structures, more PCR inhibitor residues and long fragments, etc., which is especially suitable for quantitative detection of lncRNA with relatively complex advanced structures and relatively low overall abundance.

  • Features

    ■ Highly sensitive antibody modified polymerase, suitable for fast quantitative workflow and can efficiently complete lncRNA detection.

    ■ The H-Competitor factors compete for hydrogen bonds, perfectly amplifying high GC and complex lncRNA templates.

    ■ The EP component stabilizes PCR system, effectively protects enzyme activity and resists interference of various inhibitors.

  • Applications

    It is especially suitable for experiments of lncRNA expression analysis by SYBR Green method on various real time PCR instruments, and is also suitable for quantification of templates with high GC, complex secondary structure, high impurity residue and long fragment cDNA.

  • Total RNA of Arabidopsis thaliana was extracted, and lncRNA COOLAIR was detected with TIANGEN lnRcute lncRNA First-Strand cDNA Kit+lnRcute lncRNA qPCR Kit (SYBR Green) and the reagent provided by Supplier A (RT and quantitative reagent), respectively. Amplification curve, melting curve, standard curve and Ct value are displayed. The results show that TIANGEN lncRNA specific detection reagent has high specificity and excellent gradient, and is superior to the mRNA universal reagent of Supplier A. NTC: Negative control. ND: Not detected.

  • Total RNA of Arabidopsis thaliana was extracted, and lncRNA COOLAIR was detected with TIANGEN lnRcute lncRNA First-Strand cDNA Kit+lnRcute lncRNA qPCR Kit (SYBR Green) and the reagent provided by Supplier A (RT and quantitative reagent), respectively. Amplification curve, melting curve, standard curve and Ct value are displayed. The results show that TIANGEN lncRNA specific detection reagent has high specificity and excellent gradient, and is superior to the mRNA universal reagent of Supplier A. NTC: Negative control. ND: Not detected.

  • Total RNA of human (left), mouse (middle) and rat (right) are extracted, and lncRNA (human: HEIH, mouse: NONMMUT000882, rat: NONRATT000076) was detected by TIANGEN lnRcute lncRNA First-Strand cDNA Kit+lnRcute lncRNA qPCR Kit (SYBR Green) (blue line) and the reagent provided by Supplier B (RT and quantitative reagent, red line), respectively. The amplification curve and melting curve results show that TIANGEN lncRNA specific detection reagent has high specificity and good species universality, and is superior to the mRNA general reagent of Supplier B.

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