● Highly sensitive antibody modified polymerase, suitable for fast quantitative workflow and can efficiently complete lncRNA detection.

● The H-Competitor factors compete for hydrogen bonds, perfectly amplifying high GC and complex lncRNA templates.

● The EP component stabilizes PCR system, effectively protects enzyme activity and resists interference of various inhibitors.

Compared with mRNA, lncRNA has the characteristics of low abundance, large difference in GC content and more complex secondary structure, and traditional quantitative reagents are difficult to achieve ideal effects on lncRNA. The 2×lnR lncRNA PreMix in this kit is a new generation of pre-mixed fluorescent quantitative PCR detection reagent specially developed for quantitative detection of lncRNA. The antibody modified hot-start DNA Polymerase ensures that the product has higher detection sensitivity while ensuring higher reaction specificity. In addition, the addition of H-competitor factor and EP component in Buffer makes the product have a wide range of sample universality. It has very good amplification applicability for templates with different GC contents, complex advanced structures, more PCR inhibitor residues and long fragments, etc., which is especially suitable for quantitative detection of lncRNA with relatively complex advanced structures and relatively low overall abundance.

It is especially suitable for experiments of lncRNA expression analysis by SYBR Green method on various real time PCR instruments, and is also suitable for quantification of templates with high GC, complex secondary structure, high impurity residue and long fragment cDNA.

● If the reagents are not mixed evenly, the reaction performance will be decreased. When using, please mix it by gently inverting up and down. Please do not mix it evenly with vortex, try to avoid foam, and use it after instantaneous centrifugation.

● Primer purity has a great influence on reaction specificity, so it is recommended to use primers purified above PAGE level.

● In the 20 µl reaction system, the amount of cDNA template used is generally less than 100 ng, and the amount of genomic DNA template is generally less than 50 ng. When the reverse transcription product is used as the template, the amount used should not exceed 10% of the final volume of PCR system.

Total RNA of human (left), mouse (middle) and rat (right) are extracted, and lncRNA (human: HEIH, mouse: NONMMUT000882, rat: NONRATT000076) was detected by TIANGEN lnRcute lncRNA First-Strand cDNA Kit+lnRcute lncRNA qPCR Kit (SYBR Green) (blue line) and the reagent provided by Supplier B (RT and quantitative reagent, red line), respectively. The amplification curve and melting curve results show that TIANGEN lncRNA specific detection reagent has high specificity and good species universality, and is superior to the mRNA general reagent of Supplier B.