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Methylation

Methylation-specific PCR (MSP) Kit

Methylation-specific PCR detection kit

Catalog number / packaging

Cat. no

ID

No. of preps

4992759

 EM101 400U
Manual Inquire Inspection report

  • Storage

    Store at -20℃ .

  • Description

    The product is specially developed for customers who study the methylation characteristics of genomic DNA by PCR. The kit has simple components, including 10-fold polymerase chain reaction buffer and dNTPs. Among them, MSP DNA Polymerase is a thermostable polymerase modified with antibodies, and 10×MSP PCR Buffer is a PCR buffer optimized especially for MSP reaction. It is compatible with TIANGEN DNA Bisulfite Conversion Kit (4992447).

  • Features

    ■ The product has the advantages of rapidness, simplicity, high sensitivity, strong specificity and good stability.

  • Applications

    ■ It is suitable for methylation specific PCR (MSP) method to analyze the methylation characteristics of genomic DNA.

  • Important Notes

    Do not freeze and thaw DNA templates repeatedly, as repeated freezing and thawing of DNA templates will affect amplification efficiency. If multiple experiments are needed, aliquot the templates to reduce freeze-thaw times.

  • 1: Samples processed by Supplier A; 2: Samples processed by Supplier B; 3: TIANGEN treated sample 4: NTC; M: D2000

  • 1-6: 6 repeats; 7: Supplier A processed sample; 8: NTC; Bio2-F/R and P16- Me-F/R: Two detection primers.

  • 1. Methylation-Specific PCR(MSP) Kit was applied to amplify 400 bp fragment using bisulfite treated genomic DNA as template with a reaction system of 20 μl .

  • 2. Setting of PCR reaction cycle

Publication

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