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miRNA Reagents

miRNAprep Pure FFPE Kit

Highly efficient extraction of RNA > 18 nt from paraffin sections

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Catalog Number｜Packaging

Mat. No

Ref. No

No. of preps

4992863

 GDP502 50

  • Features

    ● High specificity: Specially designed for extracting miRNA from FFPE with optimized components.

    ● Simple and fast: High-quality miRNA can be obtained within 2 hours.

    ● Ultra-pure: Column purification to avoid gDNA and salt contamination.

  • Description

    Formalin fixation can lead to cross-linking of RNA-RNA and RNA-protein in tissues, thus affecting the application of RNA in downstream experiments. The kit provides unique lysis and incubation conditions to reverse the cross-linking of RNA caused by formalin. After xylene deparaffinization, RNA can be effectively released from tissue sections by treatment with specially developed lysis buffer. The digestion of DNase I can ensure no genomic DNA contamination. RNA > 18 nt can be obtained after purification by silica matrix membrane. The product can be directly used in downstream experiments such as RT-PCR, RT-qPCR, Microarray analysis, etc.

  • Kit Contents

  • Applications

    ● RT-PCR

    ● RT-qPCR

    ● Northern Blot analysis

    ● Microarray analysis

    ● Sequencing of miRNA library

  • Required Reagents

    Xylene, anhydrous ethanol

  • Storage Condition

    DNase I, Buffer RDD and RNase-Free ddH2O (tube) should be stored at 2-8°C for 15 months. Other reagents should be stored at room temperature (15-30°C) for 15 months.

  • Important notes

    ● 96%-100% ethanol should be added to Buffer RW before using for the first time. Please refer to the label on the bottle for the added amount.

    ● Preparation of DNase I storage solution: Dissolve DNase I dry powder (1500 U) in 550 μl RNase-Free ddH2O, mix gently, and store at -30~-15°C after aliquoting (can be stored for 9 months).

    ● DNase I storage solution thawed from -30~-15°C shall be stored at 2-8°C (can be stored for 6 weeks) and shall not be frozen again.

Experimental Example

Use TIANGEN miRNAprep Pure FFPE Kit to extract miRNA of the paraffin sections of rat liver with a thickness of about 10 μm, and then sequentially carry out reverse transcription and fluorescence quantitative PCR to detect the target miRNA. A: Extract two sections and detect the expression of miR-16. Set up 2 repetitions for each section. The amplification curve shows that the kit can efficiently and stably extract miRNA in paraffin section samples. B: Use TIANGEN miRNAprep Pure FFPE Kit and relevant products from Supplier A and B to extract miRNA from the tissue sections. Each kit extracts two sections and all sections are from the same tissue sample. After extraction, perform reverse transcription and detect miR-194 expression. The experimental results in the figure show that the extraction yield of TIANGEN miRNAprep Pure FFPE Kit is slightly higher than that of similar kits of Supplier A and B.


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