miRNA Reagents
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Storage
DNase I, buffer RDD and RNase-Free ddH2O (tube) should be stored at 2-8°C. Other reagents should be stored at room temperature (15-25°C).
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Description
Formalin fixation can lead to cross-linking of RNA-RNA and RNA- protein in tissues, thus affecting the application of RNA in downstream experiments. The kit provides unique lysis and incubation conditions to reverse the crosslinking of RNA caused by formalin. After xylene deparaffinization, RNA can be effectively released from tissue sections by treatment with specially developed lysis buffer. The digestion of DNase I can ensure no genomic DNA contamination. RNA > 18 nt can be obtained after purification by silica matrix membrane. The product can be directly used in downstream experiments such as RT-PCR, RT-qPCR, Microarray analysis, etc.
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Features
■ High specificity: Specially designed for extracting miRNA from FFPE with optimized components.
■ Simple and fast: High-quality miRNA can be obtained within 2 hours.
■ Ultra-pure: Column purification to avoid gDNA and salt contamination.
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Required Reagents
Xylene, anhydrous ethanol -
Applications
■ RT-PCR
■ RT-qPCR
■ Northern Blot analysis
■ Microarray analysis
■ Sequencing of miRNA library
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