miRNA Reagents
InquireCatalog Number｜Packaging
Mat. No |
Ref. No |
No. of preps |
|
4992863 |
GDP502 | 50 |
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Features
● High specificity: Specially designed for extracting miRNA from FFPE with optimized components.
● Simple and fast: High-quality miRNA can be obtained within 2 hours.
● Ultra-pure: Column purification to avoid gDNA and salt contamination.
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Description
Formalin fixation can lead to cross-linking of RNA-RNA and RNA-protein in tissues, thus affecting the application of RNA in downstream experiments. The kit provides unique lysis and incubation conditions to reverse the cross-linking of RNA caused by formalin. After xylene deparaffinization, RNA can be effectively released from tissue sections by treatment with specially developed lysis buffer. The digestion of DNase I can ensure no genomic DNA contamination. RNA > 18 nt can be obtained after purification by silica matrix membrane. The product can be directly used in downstream experiments such as RT-PCR, RT-qPCR, Microarray analysis, etc.
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Kit Contents
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Applications
● RT-PCR
● RT-qPCR
● Northern Blot analysis
● Microarray analysis
● Sequencing of miRNA library
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Required Reagents
Xylene, anhydrous ethanol -
Storage Condition
DNase I, Buffer RDD and RNase-Free ddH2O (tube) should be stored at 2-8°C for 15 months. Other reagents should be stored at room temperature (15-30°C) for 15 months.
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Important notes
● 96%-100% ethanol should be added to Buffer RW before using for the first time. Please refer to the label on the bottle for the added amount.
● Preparation of DNase I storage solution: Dissolve DNase I dry powder (1500 U) in 550 μl RNase-Free ddH2O, mix gently, and store at -30~-15°C after aliquoting (can be stored for 9 months).
● DNase I storage solution thawed from -30~-15°C shall be stored at 2-8°C (can be stored for 6 weeks) and shall not be frozen again.
Experimental Example
Use TIANGEN miRNAprep Pure FFPE Kit to extract miRNA of the paraffin sections of rat liver with a thickness of about 10 μm, and then sequentially carry out reverse transcription and fluorescence quantitative PCR to detect the target miRNA. A: Extract two sections and detect the expression of miR-16. Set up 2 repetitions for each section. The amplification curve shows that the kit can efficiently and stably extract miRNA in paraffin section samples. B: Use TIANGEN miRNAprep Pure FFPE Kit and relevant products from Supplier A and B to extract miRNA from the tissue sections. Each kit extracts two sections and all sections are from the same tissue sample. After extraction, perform reverse transcription and detect miR-194 expression. The experimental results in the figure show that the extraction yield of TIANGEN miRNAprep Pure FFPE Kit is slightly higher than that of similar kits of Supplier A and B.
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