miRNA Reagents
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Features
● High specificity: Specially designed for extracting miRNA in serum/plasma with optimized lysis buffer.
● High efficiency: High quality miRNA can be obtained within 1 h with high extraction yield.
● Ultra-pure: Column purification to avoid gDNA and salt contamination.
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Description
miRcute Serum/Plasma miRNA Isolation Kit is a new generation of product specially developed for miRNA extraction from serum and plasma samples. The Buffer MZA in the kit has been well developed and improved, which has lysis capability and extraction sensitivity that are not available in common lysis solutions. The adsorption column in the kit adopts special silica matrix membrane filler, which greatly enhances its adsorption capability to RNA, especially small RNA(<200 nt). The extracted miRNA has better purity and higher quality, and is suitable for routine experiments such as downstream fluorescence quantification. -
Kit Contents
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Applications
The extracted product can be used in routine experiments such as RT-qPCR, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis, molecular cloning, etc.
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Storage Condition
Buffer MZA should be stored at 2-8°C protected from light for 15 months. The miRcute Serum/plasma miRNA Isolation Kit should be stored dry at room temperature (15-30°C) for 15 months.
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Important notes
To avoid RNase contamination, please note that:
● Change gloves regularly, for bacteria on the skin can result in RNase contamination.
● Use RNase-Free plastic and tips to avoid cross contamination.
● RNA can be protected in Buffer MZA. The following steps after lysis should be performed in RNase-Free plastic or glassware. To wipe off RNase, the glassware can be heated at 150°C for 4 hours, and plastic can be dipped in 0.5 M NaOH for 10 min, and washed by RNase-Free ddH2O thoroughly, then send for autoclaving.
● Use RNase-Free ddH2O to prepare the solutions. (Add DEPC to 0.1% final concentration in ddH2O. Shake to mix, and leave overnight at room temperature, then autoclave for 15 min).
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Experimental Example
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