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miRNA Reagents

miRcute Serum/Plasma miRNA Isolation Kit

High-efficiency miRNA extraction kit specifically targeting serum/plasma samples

Catalog number / packaging

Cat. no

ID

No. of preps

4992865

 DP503 50 preps
Manual Inquire Inspection report

  • Storage

    The extracted product can be used in routine experiments such as RT-qPCR, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis, molecular cloning, etc.

  • Description

    miRcute Serum/Plasma miRNA Isolation Kit is a new generation of product specially developed for miRNA extraction from serum and plasma samples. The Buffer MZA in the kit has been well developed and improved, which has lysis capability and extraction sensitivity that are not available in common lysis solutions. The adsorption column in the kit adopts special silica matrix membrane filler, which greatly enhances its adsorption capability to RNA, especially small RNA(<200 nt). The extracted miRNA has better purity and higher quality, and is suitable for routine experiments such as downstream fluorescence quantification.

  • Features

    ■ High specificity: Specially designed for extracting miRNA in serum/plasma with optimized lysis buffer.

    ■ High efficiency: High quality miRNA can be obtained within 1 h with high extraction yield.

    ■ Ultra-pure: Column purification to avoid gDNA and salt contamination.

  • Applications

    The extracted product can be used in routine experiments such as RT-qPCR, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis, molecular cloning, etc.

  • Fig. 1. miRNAs of the same sample (sample type: 200 μl human plasma) are extracted by TIANGEN miRcute Serum/Plasma miRNA Isolation Kit and Trizol precipitation extraction products of Supplier T respectively. After reverse transcription, hsa-miR-122-5p and hsa-U6 are quantitatively detected by real-time PCR respectively. The results show that the miRNA yield of plasma samples extracted by TIANGEN TIANGEN miRcute Serum/Plasma miRNA Isolation Kit is higher than that of Supplier T.

    Amplification curve: 1. U6 of the miRNA extracted by TIANGEN miRcute Serum/Plasma miRNA Isolation Kit was detected; 2. U6 of the miRNA extracted by Supplier T was detected; 3. miR-122 of the miRNA extracted by TIANGEN miRcute Serum/Plasma miRNA Isolation Kit was detected; 4. miR-122 of the miRNA extracted by Supplier T was detected.

    Reverse Transcription Reagent: TIANGEN miRcute Plus miRNA First-Strand cDNA Kit (4992786). Quantitative reagent: TIANGEN miRcute Plus miRNA qPCR Kit (SYBR Green) (4992887/4992779). Instrument: ABI 7500.


  • Fig. 2. miRNAs of the same sample (sample type: 200 μl human serum) are extracted respectively using TIANGEN miRcute Serum/Plasma miRNA Isolation Kit and relevant products of Supplier Q. After reverse transcription, hsa-miR-21-5p and miRNA detection external reference (TIANGEN CR100-01) are quantitatively detected by real-time PCR respectively. The results show that the miRNA yield of serum samples extracted by TIANGEN miRcute Serum/Plasma miRNA Isolation Kit is higher than that of similar products of Supplier Q.

    Amplification curve: 1. miRNA was extracted by TIANGEN miRcute Serum/Plasma miRNA Isolation Kit and external reference was detected; 2. miRNA was extracted by kit from Supplier Q and external reference was detected; 3. miRNA was extracted by TIANGEN miRcute Serum/Plasma miRNA Isolation Kit and miR-21 was detected; 4. miRNA was extracted by kit from Supplier Q and miR-21 was detected.

    External reference amount: 1 pmol for 200 μl serum. Reverse Reverse Transcription Reagent: TIANGEN miRcute Plus miRNA First-Strand cDNA Kit (4992786). Quantitative reagent: TIANGEN miRcute Plus miRNA qPCR Kit (SYBR Green) (4992887/4992779). Instrument: ABI 7500fast.

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