miRNA Reagents
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Features
● The lysis buffer is specially optimized and equipped with sedimentation aid solution PLA, which is specially designed for the lysis of polysaccharide polyphenol plant samples with higher yield.
● Column purification, free from impurity pollution, higher purity, suitable for downstream quantitative detection.
● Comprehensive functions to extract total RNA in addition to small fragments.
● The extraction can be completed within 1 h, improving the extraction efficiency.
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Description
This kit can rapidly extract total RNA containing miRNA from plant tissues, especially plant tissues rich in polysaccharide, polyphenol or starch (such as cotton leaves, potato tubers, apples, peach tree leaves, etc.), and can simultaneously process a large number of different samples. The extracted total RNA has high purity and is free of protein and other impurities.
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Kit Contents
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Applications
It can be used in RT-PCR, Real Time RT-PCR, chip analysis, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis, molecular cloning and other downstream experiments.
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Storage Condition
Buffer SLM added with β-mercaptoethanol can be stored at 2-8°C for one month. Other reagents should be stored at room temperature (15-30°C) for 15 months. -
Important Notes
To avoid RNase contamination, please note that:
● Change new gloves frequently, because the skin contains bacteria and may cause RNase contamination.
● Use RNase-free plastic products and pipette tips to avoid cross contamination.
● RNA will not be degraded by RNase in Buffer SLM. However, plastic and glassware without RNase should be used in the subsequent processing.
● RNase-Free ddH2O should be used for the preparation of solutions (add water into a clean glass bottle, add DEPC to a final concentration of 0.1%(v/v), mix well, place overnight, and autoclave).
Precautions Please read these precautions before use：
● Before operation, add β-mercaptoethanol to Buffer SLM to a final concentration of 5%. For example, add 25 μl of β-mercaptoethanol to 475 μl of Buffer. It’s better to prepare the reagent when using. The prepared reagent can be stored for one month at 2-8°C.
● Add 96-100% ethanol to Buffer MRD and Buffer RWA before the first use. Please refer to the label on the bottle for the added volume.
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Experimental Example
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