miRNA Reagents

miRcute Plant miRNA Isolation Kit

High-efficiency miRNA extraction kit specially targeting plant samples

Catalog number / packaging

Cat. no

ID

No. of preps

4992733

DP504 50 preps
  • Storage

    Store at room temperature (15-25°C).
  • Description

    This kit can rapidly extract total RNA containing miRNA from plant tissues, especially plant tissues rich in polysaccharide, polyphenol or starch (such as cotton leaves, potato tubers, apples, peach tree leaves, etc.), and can simultaneously process a large number of different samples. The extracted total RNA has high purity and is free of protein and other impurities.

  • Features

    ■ The lysis buffer is specially optimized and equipped with sedimentation aid solution PLA, which is specially designed for the lysis of polysaccharide polyphenol plant samples with higher yield.

    ■ Column purification, free from impurity pollution, higher purity, suitable for downstream quantitative detection.

    ■ Comprehensive functions to extract total RNA in addition to small fragments.

    ■ The extraction can be completed within 1 h, improving the extraction efficiency.

  • Applications

    It can be used in RT-PCR, Real Time RT-PCR, chip analysis, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis, molecular cloning and other downstream experiments.

  • Figure. 1. Use Trizol reagent from Supplier A (A) and TIANGEN miRcute Plant miRNA Isolation Kit (T) to extract total RNA from 50 mg corn kernels and 50mg cotton leaves respectively, and the extraction effect is detected by electrophoresis. The results show that for plant samples rich in polysaccharide/polyphenol, the extraction efficiency of RNA by TIANGEN miRcute Plant miRNA Isolation Kit is higher than that by Trizol products.

    M: Marker

  • Figure. 2. Use TIANGEN miRcute Plant miRNA Isolation Kit and relevant products from Supplier B to extract total RNA and miRNA from 50 mg cotton leaves, and the RNA was reverse transcribed by TIANGEN miRcute Plus miRNA First-Strand cDNA Kit (4992786), and MIR166a was detected by TIANGEN miRcute Plus miRNA qPCR Kit (SYBR Green) (4992887/4992779). The amplification curve (A) and melting curve (B) show that, for plant samples rich in polysaccharide/polyphenol, TIANGEN miRcute Plant miRNA Isolation Kit has high overall yield and good purity, which is more suitable for downstream quantitative detection.

    Blue line: Total RNA with miRNA extracted with TIANGEN miRcute Plant miRNA Isolation Kit

    Red line: Total RNA with miRNA extracted with product of Supplier B

    Yellow line: miRNA extracted with TIAGEN miRcute Plant miRNA Isolation Kit

    Green line: miRNA extracted with product of Supplier B