The research of bacterial genome mainly includes DNA extraction and sequencing, genome assembly, gene prediction, gene annotation, genome comparison and analysis, etc. Based on different study purpose, different schemes, such as NGS、Pacbio sequencing or Oxford Nanopore sequencing, can be selected to achieve bacterial genome assembly of different degree, thus laying a foundation for more detailed and in-depth research of target strains. 

Scheme design → Sample processing → Library construction → Sequencing → Data analysis → After-sales service 

Circos diagram of gene and annotation results 

In order to visually display the results of gene annotation, we made a doughnut diagram of the genome by CIRCOS. It includes GC content, GC offset, positive and negative strand genes, rRNA, tRNA prediction and other information.

CRISPR sequence prediction 

CRISPR is composed of repeat sequences and spacer sequences with similar length. It is the central component of the bacterial immune system and is responsible for sequence recognition. It also plays an important role in the bacterial immune system. It can resist the invasion of foreign DNA such as phage and plasmid. We use CRT to predict CRISPR sequence. The results could not only locate the position of CRISPR on the genome, but also include repeat sequences and spacer sequences. 

COG notes

COG (Cluster of Orthologous Groups of proteins) is an NCBI classified database based on gene lineal homology. Combined with evolutionary relationships, COG classifies homologous genes from different species into different ortholog clusters. Currently, the COG has 4873 categories. Genes from the same ortholog have the same function, so functional annotations can be directly passed to other members of the same COG cluster.