Bacterial and fungal whole genome re-sequencing is a critical tool to complete the genomes of known bacterium and fungi, includes third generation sequencing, assembly, functional annotation and advanced bioinformatic analysis fulfilling specific research goals. A more comprehensive profiling of bacteria genome empowers revealing of fundamental mechanisms underlying their biological processes, which could also provide valuable reference for genomic researches in higher eukaryotic species, etc.

Scheme design → Sample processing → Library construction sequencing → Data analysis → After-sales service 

Industrial field, human microbiology, environmental microbiology, agricultural field 

Circos diagram of gene and annotation results 

In order to visually display the results of gene annotation, we made a doughnut diagram of the genome by CIRCOS. It includes GC content, GC offset, positive and negative strand genes, rRNA, tRNA prediction and other information. 

CRISPR sequence prediction 

CRISPR is composed of repeat sequences and spacer sequences with similar length. It is the central component of the bacterial immune system and is responsible for sequence recognition. It also plays an important role in the bacterial immune system. It can resist the invasion of foreign DNA such as phage and plasmid. We use CRT to predict CRISPR sequence. The results could not only locate the position of CRISPR on the genome, but also include repeat sequences and spacer sequences.

COG notes 

COG (Cluster of Orthologous Groups of proteins) is an NCBI classified database based on gene lineal homology. Combined with evolutionary relationships, COG classifies homologous genes from different species into different ortholog clusters. Currently, the COG has 4873 categories. Genes from the same ortholog have the same function, so functional annotations can be directly passed to other members of the same COG cluster.