16S/18S/ITS Amplicon Metagenomic Sequencing detects gene fragments in specific regions to obtain information about microbial diversity and community composition differences of bacteria/eukaryotic microbes/fungi other species in the sample. It can be divided into DNA extraction, PCR amplification, library construction, sequencing, data analysis and other steps. 

Scheme design → Sample processing → Library construction sequencing → Data analysis → After-sales service 

Industrial field, human microbiology, environmental microbiology, agricultural field 

Sequencing depth analysis of single sample 

It is used to assess whether the sequencing data volume of each sample is sufficient to cover all groups in the community and indirectly reflects the species richness in the sample. When the observed_species curve tends to be gentle, it can be assumed that the sequencing depth covers almost all species in the sample. On the contrary, it indicates that the species diversity in the sample is higher, and there are more undetected species, so more reads need to be measured to meet the analysis requirements.

Community structure analysis of multi-sample species 

The software RDP classifier was used to annotate species with GreenGenes database, and the species composition of each sample at different classification levels was counted to draw a community map of multi-sample species composition. Unknown indicates that the abundance of this classification level cannot be determined. Only some species with higher abundance are shown in the figure, and the species with abundance lower than 1% are shown as Others.

Association network analysis of microbial species  

The fundamental objective of association network analysis of microbial species is to investigate the interaction between membersof different communities, and to look for the interactive model of Co-occurrence or Co-exclusion of microbial species in different habitats through association analysis, so as to infer the possible "cooperation" or "competition" relationship between different microbial members.