PCR Systems
InquireCatalog Number｜Packaging
Mat. No |
Ref. No |
No. of preps |
|
OSE-GP-03-AM |
OSE-GP-03-AM | 1 Set |
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Overview
The TGreat Expert thermal cycler is a new-generation high-performance gradient PCR system designed for precise DNA amplification in routine molecular biology workflows. With advanced gradient temperature control, high accuracy, and stable performance, it enables reliable optimization and reproducible results for everyday PCR applications.
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Why Choose This PCR Thermal Cycler
- Consistent performance across wells – reliable amplification across all 96 wells
- Fast ramp rate – reduces total experiment time for routine PCR workflows
- Wide gradient capability – simplifies optimization in a single run
- Easy-to-use system – intuitive operation for everyday laboratory use
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Features
- Fast ramp rate up to 5°C/s – reduces experiment cycle time and improves workflow efficiency
- High temperature accuracy (±0.1°C) – ensures reliable and reproducible PCR results
- Wide gradient range (1–42°C) – simplifies optimization of annealing conditions
- 10.1” capacitive touchscreen – intuitive interface for fast and easy operation
- Adjustable ramp rate (0.1–5°C/s) – flexible settings for diverse PCR protocols
- Built-in smart tools – Tm calculator and gradient simulator to streamline experiment setup
- WiFi connectivity with email alerts – enables real-time monitoring and notification
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Applications
- Routine PCR and DNA amplification
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Gene cloning and molecular biology research
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Pre-PCR sample preparation for qPCR workflows
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PCR optimization including gradient PCR, Touchdown PCR, and long PCR
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Specifications
Technical Specifications
Parameter TGreat Expert Sample Capacity 96 × 0.2 ml tubes / 96-well plate Temperature Range 0–105°C Maximum Ramp Rate 5°C/s Temperature Accuracy ±0.1°C Temperature Uniformity ±0.2°C Gradient Range 1–42°C Heated Lid Temperature 30–115°C Interface & Connectivity 10.1” touchscreen, USB & WiFi -
FAQ
What is a 384‑well PCR thermal cycler?
A: A 384‑well PCR thermal cycler is a high‑throughput system designed to perform up to 384 reactions simultaneously for large‑scale molecular biology experiments.Why use a 384‑well PCR thermal cycler?
A: It increases sample throughput, improves laboratory efficiency, and reduces overall experiment time, making it ideal for high‑throughput PCR workflows.How is a 384‑well PCR thermal cycler different from a 96‑well system?
A: A 384‑well system supports larger‑scale experiments and higher throughput, enabling efficient batch processing. For routine workflows, a 96‑well PCR thermal cycler may be more suitable.Is a 384‑well PCR thermal cycler suitable for routine use?
A: It is optimized for high‑throughput and batch experiments, rather than routine small‑scale laboratory workflows.Does this PCR system support gradient PCR?
A: Yes, it supports gradient PCR with a wide temperature range, allowing efficient optimization of annealing conditions. -
PCR Thermal Cycler Comparison
Compare different PCR thermal cyclers to identify the optimal system for your workflow.
Technical Specifications
Block / TubeBlock / TubeBlock / TubeParameter 96‑Well 384‑Well Dual‑Block Product Model TGreat Expert (96 well) TGreat Expert (384 well) TGreat Expert (dual‑slot) Sample Capacity 96 × 0.2 ml 384 well plate 64 + 32 × 0.2 ml Consumable 0.2 ml tubes, 8‑strip, 96‑well plate 384 well microplate 0.2 ml tubes, 8‑strip Volume 1–100 μl 1–50 μl 1–120 μl Temperature Range 0–105°C 0–105°C 0–105°C Maximum Ramp Rate 5°C/s 5°C/s 5°C/s Temperature Uniformity ≤ ±0.2°C ≤ ±0.2°C ≤ ±0.2°C Temperature Accuracy ≤ ±0.1°C ≤ ±0.1°C ≤ ±0.1°C Temperature Control Mode Block / Tube Block / Tube Block / Tube Adjustable Ramp Rate 0.1–5°C/s 0.1–5°C/s 0.01–5°C/s Gradient Setting Range 1–42°C 1–42°C 1–30°C Gradient Distribution 12‑column gradient 24‑column gradient 8‑column per block Maximum Program Steps 30 30 40 Display 10.1”, 1024×600 10.1”, 1024×600 10.1”, 1280×800
Experimental Example
Experimental Validation
The TGreat Expert thermal cycler series utilizes a unified advanced thermal control platform, ensuring consistent performance across both 96‑well and 384‑well configurations.
Temperature Uniformity Across Wells
Temperature uniformity was evaluated by amplifying a 1.5 kb DNA fragment across selected well positions using touchdown PCR. The results demonstrate uniform amplification across all tested wells, confirming excellent temperature consistency and reliable performance.
- Uniform band intensity across wells indicates stable temperature control
- Minimal non‑specific amplification ensures high specificity
- Consistent results support reproducibility in routine laboratory workflows
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