PCR Systems
InquireCatalog Number｜Packaging
Mat. No |
Ref. No |
No. of preps |
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OSE -GP-05-AM |
OSE -GP-05-AM | 1 Set |
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Overview
The TGreat Expert (384-well) Gradient Thermal cycler is a high‑performance system designed for high‑throughput DNA amplification and large‑scale PCR workflows. Its advanced gradient temperature control, excellent uniformity, and fast ramping capability enable efficient optimization and consistent results across large sample sets.
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Why Choose This PCR Thermal Cycler
- High‑throughput processing up to 384 reactions per run
- Excellent temperature uniformity – ensures consistent results across all wells
- Fast and efficient workflow – enables large‑scale PCR experiments
- Reduced reagent usage – improves cost efficiency in batch processing
For routine experiments, a 96-well PCR thermal cycler may be more suitable.
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Key Features
- High‑speed ramp rate up to 5°C/s – accelerates large‑scale experiments
- Excellent temperature uniformity (≤ ±0.2°C) – ensures consistent results across all wells
- Wide gradient range (1–42°C) – efficient optimization in one run
- 24‑column gradient design – supports high‑throughput optimization
- WiFi and remote monitoring – real‑time tracking and control
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Applications
- High‑throughput PCR screening
- Large‑scale DNA amplification and gene analysis
- PCR‑based pathogen detection workflows
- NGS sample preparation workflows
- Multi‑sample PCR optimization and amplification
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Specifications
384‑Well PCR Thermal Cycler Technical Specifications
Parameter TGreat Expert (384) Sample Capacity 384 wells Temperature Range 0–105°C Maximum Ramp Rate 5°C/s Temperature Accuracy ±0.1°C Temperature Uniformity ≤ ±0.2°C Gradient Range 1–42°C Gradient Distribution 24‑column gradient (left to right) Heated Lid Temperature 30–115°C Display 10.1” touchscreen Interface & Connectivity USB 2.0, WiFi Program Storage 20000+ (USB supported) Dimensions (L×W×H) 385 × 270 × 255 mm Weight 10 kg Power Supply 100–240 V, 50/60 Hz, 750 W -
FAQ
What is a 384‑well gradient PCR thermal cycler?
A: A 384‑well gradient PCR thermal cycler is a high‑throughput system designed to perform up to 384 reactions simultaneously with gradient temperature control for efficient optimization.Why choose a 384‑well PCR thermal cycler?
A: It enables large‑scale PCR experiments, increases sample throughput, and improves laboratory efficiency for screening and batch processing workflows.How is a 384‑well PCR system different from a 96‑well PCR cycler?
A: A 384‑well system provides significantly higher throughput and supports large‑scale experiments, while 96‑well systems are more suitable for routine laboratory workflows.Does the 384‑well PCR system support gradient PCR?
A: Yes, it features a wide gradient temperature range, allowing simultaneous optimization of PCR conditions across multiple samples.What applications is a 384‑well PCR thermal cycler best suited for?
A: It is ideal for high‑throughput PCR screening, large‑scale gene analysis, pathogen detection, and NGS sample preparation workflows. -
PCR Thermal Cycler Comparison
Compare different PCR thermal cyclers to identify the optimal system for your workflow.
Technical Specifications
Block / TubeBlock / TubeBlock / TubeParameter 96‑Well 384‑Well Dual‑Block Product Model TGreat Expert (96 well) TGreat Expert (384 well) TGreat Expert (dual‑slot) Sample Capacity 96 × 0.2 ml 384 well plate 64 + 32 × 0.2 ml Consumable 0.2 ml tubes, 8‑strip, 96‑well plate 384 well microplate 0.2 ml tubes, 8‑strip Volume 1–100 μl 1–50 μl 1–120 μl Temperature Range 0–105°C 0–105°C 0–105°C Maximum Ramp Rate 5°C/s 5°C/s 5°C/s Temperature Uniformity ≤ ±0.2°C ≤ ±0.2°C ≤ ±0.2°C Temperature Accuracy ≤ ±0.1°C ≤ ±0.1°C ≤ ±0.1°C Temperature Control Mode Block / Tube Block / Tube Block / Tube Adjustable Ramp Rate 0.1–5°C/s 0.1–5°C/s 0.01–5°C/s Gradient Setting Range 1–42°C 1–42°C 1–30°C Gradient Distribution 12‑column gradient 24‑column gradient 8‑column per block Maximum Program Steps 30 30 40 Display 10.1”, 1024×600 10.1”, 1024×600 10.1”, 1280×800
Experimental Example
Experimental Validation
The TGreat Expert thermal cycler series shares a unified high‑precision thermal control platform, ensuring reliable and consistent performance across different configurations, including high‑throughput 384‑well systems.
Temperature Uniformity for High‑Throughput PCR
Temperature uniformity was validated by amplifying a 1.5 kb DNA fragment across multiple well positions. The results demonstrate consistent amplification across a large number of wells, confirming excellent temperature uniformity for high‑throughput applications.
- Consistent band intensity across multiple wells
- Excellent temperature uniformity ensures reproducibility at scale
- Reliable performance for batch processing and high‑throughput workflows
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