Single Column
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Features
● Silica-membrane technology provides fast way to purify ready-to-use high quality gDNA.
● High DNA yield, excellent repeatability.
● This kit significantly removes contaminants and inhibitors, facilitating downstream applications.
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Description
The TIANamp Genomic DNA Kit provides a fast and easy silica-based format for DNA purification. The kit simplifies the purification of DNA from a wide range of sample types, including animal blood (≤ 1 ml), cultured cells, and tissues commonly encountered in life science, veterinary, and genotyping applications. Most samples could be directly lysed with the provided buffer system, eliminating the need for mechanical disruption and also reducing hands-on time. The kit purifies high-quality DNA without proteins and inhibitors. The purified DNA is suitable for PCR, restriction digestion and Southern blot.
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Kit Contents
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Required Reagents
Red cell lysis buffer (for blood samples more than 200 μl, Cat# 4992810), ethanol, RNase A (optional) -
Yield For Reference
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Storage Condition
TIANamp Genomic DNA Kit can be stored dry at room temperature (15-30°C) for up to 15 months without showing any reduction in performance and quality. If any precipitate forms in the buffers, it should be dissolved by warming the buffers at 37°C for 10 min before use. -
Important Notes
● Please use fresh samples to ensure the integrity of the purifed gDNA.
● If the sample starting amount exceeds the recommended range of the kit, it is recommended to increase the amount of buffer GA and buffer GB, and split the column loading in separate times, or perform the experiment using two spin columns.
● For long-term storage of the gDNA, it is recommended to store in buffer TE. Please ensure that the OD260/OD280 value is in the range of 1.7-1.9, and the concentration of gDNA is 100-300 ng/μl.
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Certification
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